Effects of basic fibroblast growth factor modified skeletal muscle satellite cell transplantation on neovascularization following acute myocardial infarction

doi:10.3969/j.issn.2095-4344.2017.09.016

Abstract

Abstract:

BACKGROUND: Previous studies have found that skeletal muscle satellite cell transplantation can induce angiogenesis in myocardial infarction area, reduce infarct size and improve cardiac function. But the overall effect is not satisfactory.
OBJECTIVE: To observe the survival of basic fibroblast growth factor (bFGF) gene modified skeletal muscle satellite cells in acute myocardial infarction and to observe the expression of bFGF gene and the effect of cell transplantation on angiogenesis in myocardial infarction area.
METHODS: Eighteen New Zealand white rabbits were divided into three groups by random: skeletal muscle satellite cell group (control group), bFGF gene enhanced skeletal muscle satellite cell group (experimental group) and blank control group. The left anterior descending branch of the coronary artery of the rabbits was ligated so as to establish an animal model of acute myocardial infarction in the former two groups. After labeled by DAPI before transplantation, the skeletal muscle satellite cells, bFGF gene modified skeletal muscle satellite cells and the equivalent amount of DMEM/F12 were injected into the local infarct myocardium correspondingly. Samples were taken 4 weeks after transplantation. Then, the survival of skeletal muscle satellite cells and the expression of bFGF gene were observed under light microscope and fluorescence microscope, and the neovascularization in the myocardial infarction area was examined by immunohistochemical staining.
RESULTS AND CONCLUSION: No DAPI-labeled cells were visible in the blank control group, but in the other two groups, a large amount of DAPI-labeled skeletal muscle satellite cells were seen in the infarction area. Enhanced green fluorescent protein was highly expressed in the experimental group. Microvessel density in the infarction area was highest in the experimental group followed by the control and blank control groups (P < 0.05). These findings indicate that bFGF gene modified skeletal muscle satellite cells can survive and promote neovascularization in the acute myocardial infarction area.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Satellite Cells, Skeletal Muscle, Fibroblast Growth Factors, Cell Transplantation, Gene Therapy, Tissue Engineering

CLC Number:

R394.2

Xu Zhi-feng, Li Jing-lai, Han Zhen, Feng Gang, Ren Ming-ming. Effects of basic fibroblast growth factor modified skeletal muscle satellite cell transplantation on neovascularization following acute myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(9): 1397-1401.

Figures/Tables 1

References

[1] Mesires NT,Doumit ME.Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle.Am J Physiol Cell Physiol. 2002;282(4):C899-906.

[2] Fujimaki S,Machida M,Hidaka R,et al.Intrinsic ability of adult stem cell in skeletal muscle: an effective and replenishable resource to the establishment of pluripotent stem cells.Stem Cells Int.2013;2013:420-424.

[3] Kao GW,Lamb EK,Kao RL,et al.Skeletal muscle stem cells. Methods Mol Biol. 2013;1036:19-32.

[4] 王立新,高长青.自体骨骼肌星状细胞心肌成形的实验研究[J].中华医学杂志,2002,82(2):100-103.

[5] Ye L,Haider HK,Tan R,et al.Angiomyogenesis using liposome based vascular endothelial growth factor-165 transfection with skeletal myoblast for cardiac repair. Biomaterials.2008; 29(13):2125-2137.

[6] Usas A,Huard J.Muscle-derived stem cells for tissue engineering and regenerative therapy. Biomaterials. 2007; 28(36):5401-5406.

[7] 徐青,徐敏,张加吉,等.壁虎骨骼肌卫星细胞的原代培养与鉴定[J].交通医学,2011,25(2):110-112.

[8] 许志锋,李敬来,韩振,等.阳离子脂质体介导增强型绿色荧光蛋白质粒转染骨骼肌卫星细胞[J].中国组织工程研究,2013,17(46): 86-91.

[9] Swedlow JR,Goldberg l,Brauner E,et al.Informatics and quantitative analysis in biological imaging.Science.2003; 300(5616):100-102.

[10] 李国营,田素民,于连发,等.兔心肌缺血及缺血再灌注模型的制备[J].解剖学研究,2004,24(3):237-238.

[11] 蒋清安,何国祥,刘建平,等.一种新的非人工通气兔心肌梗死模型的制作[J].第三军医大学学报,2008,30(5):406-409.

[12] 何智明,陈东风,邹香云,等.家兔心肌缺血模型与冠状动脉左室支解剖特点关系[J].中国临床解剖学杂志,1994,12(1):54-56.

[13] Minhajat R,Mori D,Yamasaki F,et al.Organ-specific endoglin(CD105) expression in the angiogenesis of human cancers.Pathol Int.2006;56(12):717-723.

[14] Weidner N.Current pathologic methods of measuring intratumoralmicrovessel density within breast carcinoma and other solidertumors.Breast Cancer Res Treat. 1995;36(1): 169-180.

[15] 倪玉霞,刘小青,李贻奎,等.骨髓间充质干细胞移植联合冠心Ⅱ号对大鼠急性心肌梗死心功能和血管新生的影响[J].中国组织工程研究与临床康复,2008,12(47):9201-9209.

[16] Muñoz-Cánoves P,Carvajal JJ,Lopez de Munain A et al.Role of Stem Cells in Skeletal Muscle Development, Regeneration, Repair, Aging, and Disease. Front Aging Neurosci.2016;8:95.

[17] Arnold HH,Winter B.Muscle differentiation: more complexity to the network of myogenic regulators.Curr Opin Genet Dev. 1998; 8:539-544.

[18] Peault B,Rudnicki M,Torrente Y,et al.Stem and progenitor cells in skeletal muscle development, maintenance, and therapy.Mol Ther.2007;15:867-877.

[19] Mesires NT,Doumit ME.Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle.Am J Physiol Cell Physiol.2002;282:C899-906.

[20] Seidel M,Borczyńska A,Rozwadowska N,et al.Cell-based therapy for heart failure: skeletal myoblasts.Cell Transplant. 2009;18(7):695-707.

[21] Nakamura Y,Asakura Y,Piras B,et al.AIncreased angiogenesis and improved left ventricular function after transplantation of myoblasts lacking the MyoD gene into infarcted myocardium.PLoS One.2012;7(7):417-436.

[22] Shudo Y, Miyagawa S, Ohkura H et al. Addition of mesenchymal stem cells enhances the therapeutic effects of skeletal myoblast cell-sheet transplantation in a rat ischemic cardiomyopathy model.Tissue Eng Part A.2014;20(3-4): 728-739.

[23] Sanganalmath SK,Bolli R.Cell therapy for heart failure: a comprehensive overview of experimental and clinical studies, current challenges, and future directions.Circ Res.2013; 113(6):810-834.

[24] House SL,Castro AM,Lupu TS,et al.Endothelial fibroblast growth factor receptor signaling is required for vascular remodeling following cardiac ischemia-reperfusion injury.Am J Physiol Heart Circ Physiol.2016;310(5):H559-571.

[25] Zhao T,Zhao W,Chen Y,et al.Acidic and basic fibroblast growth factors involved in cardiac angiogenesis following infarction.Int J Cardiol.2011;152(3):307-313.