Abstract:

Abstract
BACKGROUND: Insufficient blood supply after major bone defects leads to osteocyte necrosis; therefore, the tissue-engineered bone cannot meet the clinical requirements.
OBJECTIVE: To construct an artificial vessel using in vitro cultured rabbit bone marrow stromal stem cells and human umbilical vein endothelial cells combined with the rabbit aortic decellularized vascular matrix.
METHODS: Rabbit bone marrow stromal stem cells were cultured by adherent method, human umbilical vein endothelial cells were cultured in vitro and isolated, and rabbit aortic decellularized vascular stent was prepared. The stent-cell composite was constructed, and its biocompatibility was observed under scanning electron microscope. There were three groups: blank control, control (bone marrow stromal stem cell-stent) and experimental (stent-cell composites) groups (n=10 per group), followed by implantation around the small vessels. Three months later, the vascular samples were collected for gross observation and hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Fibroblast-like cell clones were formed by adherent method, and were positive for CD44+ and STRO-1+ after climbing; calcified nodule formed in the ossification. Flow cytometry showed CD34+ accounting for 43.83%, and visible VIII factor in the endothelial cells. In the experimental group, endothelial cells formed a single cell layer onto the scaffold under scanning electron microscope; the artificial vessel grew well and tubular structure presented and eight angiod structures were found. Thin film-like vessels formed in the blank control group and there were only endothelial-like tissues in the control group. These results suggest that angiod structures can form after the stent-cell composite implantation, causing no immune reaction, but the artificial vessel is visible in absence of muscular layer.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Stem cells, Endothelial Cells, Blood Vessel Prosthesis, Tissue Engineering