Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (52): 7815-7820.doi: 10.3969/j.issn.2095-4344.2016.52.009

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In vitro study on a degradable poly-L-lactic acid biliary-enteric composite stent

Yao Jin-feng1, Han Jin-li2, Guo Yu1, Cui Dong-lai1 
  

  1. 1Department of Gastroenterology, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China; 2Department of Endoscope, Boding Seven Hospital, Baoding 072150, Hebei Province, China
  • Received:2016-09-24 Online:2016-12-16 Published:2016-12-16
  • Contact: Han Jin-li, Attending physician, Department of Endoscope, Boding Seven Hospital, Baoding 072150, Hebei Province, China
  • About author:Yao Jin-feng, M.D., Chief physician, Department of Gastroenterology, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
  • Supported by:

    the Key Medical Science Research Project in 2012, No. 201200817

Abstract:

BACKGROUND: The degradable poly-L-lactic acid (PLLA) biliary-enteric composite stent has been developed.
OBJECTIVE: To analyze the solubility and biocompatibility of the degradable PLLA biliary-enteric composite stent.
METHODS: Solubility: the PLLA composite stent was implanted into artificial gastric acid to detect the dissolution rate within 12 weeks. Pyrogen test: the PLLA composite stent extracts were injected into the rabbits via ear vein to detect the changes of body temperature. Hemolysis test: the PLLA composite stent extracts, normal saline and distilled water were added into the rabbit anticoagulant, respectively, to detect the hemolysis ratio. Cytocompatibility test: Caco-2 cells were respectively cultured in the DMEM medium containing 10% fetal bovine serum, rubber material extracts and the PLLA composite stent extracts, and the cell proliferation was detected at 12, 24, 36 and 48 hours; the lactic dehydrogenase release was detected at 2 days.
RESULTS AND CONCLUSION: The PLLA composite stent showed a long stability in vivo, and approximately 80% was dissolved at about 20 weeks. No reactions of pyrogen and henolysis were observed in the pyrogen and hemolysis tests. The PLLA composite stent made no effects on the Caco-2 cell proliferation and lactic dehydrogenase release. In conclusion, the PLLA composite material holds a good solubility and cytocompatibility.

Key words: Biocompatible Materials, Lactate Dehydrogenases, Tissue Engineering

CLC Number: