Platelet-rich plasma promotes bone mineralization during distraction osteogenesis

doi:10.3969/j.issn.2095-4344.2016.46.005

Abstract

Abstract:

BACKGROUND: The platelet-rich plasma contains various growth factors that could enhance the regeneration of certain tissues.
OBJECTIVE: To observe the effect of platelet-rich plasma on the mineralization process during distraction osteogenesis in rabbits.
METHODS: Thirty-two adult Japanese white rabbits were randomly divided into four groups (n=8 per group). The upper 1/3 tibial osteotomy at the posterior limb was performed in all rabbits, and the 1 cm limb lengthening models were prepared, followed by the local injection of 500 μL platelet-rich plasma at 5, 15 and 25 days. The controls received no treatment. At 37 days after modeling, the rabbits were killed and newly formed callus was removed to undergo hematoxylin-eosin staining, and the frontal X-ray film of tibia was obtained.
RESULTS AND CONCLUSION: Lane-Sandhu radiographic scoring showed that the scores in the injection groups at 15 and 25 days after modeling were obviously better than those in the control group and the injection at 5 days after modeling. Lane-Sandhu histological scoring revealed that the scores in the injected groups at 15 and 25 days after modeling were significantly higher than those in the control group and group at 5 days after modeling (P < 0.05), which highest at 25 days. These results suggest that local injection of platelet-rich plasma can promote bone mineralization during distraction osteogenesis effectively, especially in the middle period of distraction osteogenesis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Platelet-Rich Plasma, Bone Lengthening, Tissue Engineering

CLC Number:

R318

Shi Bo-wen, Li Da-wei, Gong Xian-bin, Ji Guo-qi, Feng Yuan-chao, Cai Cheng-kuo, Shu Heng-sheng. Platelet-rich plasma promotes bone mineralization during distraction osteogenesis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(46): 6878-6884.

Figures/Tables 2

度高于其他各组；术后第35天，各组新生骨密度较第25天明显增高，其中以建模后15 d注射组、建模后25 d注射组牵拉成骨区出现大量纵行排列的条索状或片状的影像，骨痂密度较高，而对照组、建模后5 d注射组延长区仍存在少量透光区域(图2)。按照Lane-Sandhu X射线评价标准评分，各组评分结果及统计结果见表1，2。统计结果显示，建模后15 d注射组、建模后25 d注射组Lane-SandhuX线评分明显高于对照组、建模后5 d注射组(P < 0.05)，差异有显著性意义；建模后25 d注射组平均得分16.0高于建模后15 d注射组(11.25)，且二者之间差异有显著性意义(P < 0.05)；对照组、建模后5 d注射组两两比较，差异无显著性意义(P > 0.05)。 2.4 组织学观察空白对照组组织肿胀明显，视野中存在较多纤维组织，骨小梁结构少，未出现哈弗斯管系统及软骨化骨。建模后5 d注射组软骨化骨不活跃，成骨细胞数目较少，骨小梁密度也较低，大量纤维组织填充间隙；建模后15 d注射组软骨化骨不活跃，成骨细胞明显增多，视野中出现低成熟度的骨小梁，但结构致密；建模后25 d注射组成骨行为活跃，出现多个软骨化骨中心，并出现成熟度较高的哈弗斯管系统，骨重塑活跃，骨小梁排列致密，成骨细胞数目丰富，骨陷窝细胞较多(图3，4)。按照Lane-Sandhu组织学评价标准评分，各组评分及统计结果见表3，4。结果显示，建模后15 d注射组、建模后25 d注射组Lane-Sandhu组织学评分明显优于对照组、建模后5 d注射组，差异有显著性意义(P < 0.05)；建模后25 d注射组组织学评分平均分值最高(12.38)，与建模后15 d注射组比较，差异有显著性意义(P < 0.05)；对照组、建模后5 d注射组之间两两比较，差异无显著性意义(P > 0.05)。"

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