Comparison of physicochemical properties of five kinds of cancellous bones

doi:10.3969/j.issn.2095-4344.2016.42.001

Abstract

Abstract:

BACKGROUND: Existing evidence has shown that xenogeneic bone derived graft materials have the ability to repair bone defects, but little report is on the comparison of composition of xenogeneic bone and human bone.
OBJECTIVE: To analyze the chemical composition, mineral phase, calcium and phosphorus contents, total protein contents, and appearance of the cancellous bones of the tibia from pig, bovine and human.
METHODS: Cancellous bones of the tibia from pig, Banna miniature pig, bovine, young and old people were cut into bone particles of 0.5 cm×0.5 cm×0.5 cm. After degreasing or drying treatment, chemical composition, mineral phase, calcium and phosphorus contents, total protein contents of bone particles were detected using Fourier transform infrared spectroscopy, X-ray diffraction, plasma emission spectroscopy, scanning electron microscopy, and Kjeldahl method, respectively. Nano Measurer 1.2 and Photoshop cs6 were used to measure pore diameter and porosity rate based on the results of scanning electron microscope.
RESULTS AND CONCLUSION: The main inorganic phase of five kinds of cancellous bones was hydroxyapatite and the main chemical composition was phosphate and carbonate. The calcium content of the bovine cancellous bonewas lower than that of the other cancellous bones (P < 0.05) and the phosphorus content of the cacellous bone of young man was higher than that of the pig and bovine cancellous bones (P < 0.01). The total protein content in the cancellous bones of normal pig and Banna miniature pig was higher than that in the other three kinds of cancellous bones (P < 0.05). The pore diameter of five kinds of cancellous bones was 400 to 600 μm and the porosity rate was 60% to 70%. Moreover, the porosity rate of the bovine cancellous bone was the highest. Therefore, we conclude that there are some certain differences in the organic and inorganic components of the pig or bovine cancellous bones as compared with humans.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Scaffolds, Bone Transplantation, Tissue Engineering

CLC Number:

Gao Huan-yu, Li Yan-lin, Xiao Yu, Li Long-teng, Wang Guo-liang. Comparison of physicochemical properties of five kinds of cancellous bones[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(42): 6237-6243.

Figures/Tables 1

2.1 五种松质骨大体观 5种新鲜松质骨的横截面有大小不等孔隙，且孔隙中有骨髓和油脂填充，牛松质骨为乳白色，普通猪与版纳微型猪为粉红色，老人和年轻人松质骨为褐黄色，见图1。 2.2 五种松质骨的红光检测结果由图2可见，5种松质骨的出峰位置基本一致，说明5种松质骨中的化学成分大致相同，在3 428 cm-1 处宽化的中强峰由样本中水成分引发；2 925，2 856，1 747 cm-1处的强峰分别由脂类中的C-H键的不对称伸缩振动(νas)、C-H键的对称伸缩振动(νs)与-COOH的脂肪酮键的伸缩振动(νc=o)引起；1 600-1 700 cm-1段与1 500-1 600 cm-1段的弱峰分别归结为代表有机物(主要是蛋白质)Ⅰ级酰胺、Ⅱ级酰胺，其中酰胺Ⅰ主要由C=O的伸缩振动引起，酰胺Ⅱ由N-H弯曲振动与C-N 伸缩振动引发；在1 630 cm-1处亦有水的振动峰。1 458，875，719 cm-1的振动峰为碳酸根中的不同的C-O键的伸缩振动引起；875 cm-1波段处主要为HPO42-的基本振动出峰位置；900-1 200 cm-1段主要反应骨中无机成分中的PO43-成分，同时在这一波段中1 244 cm-1处的振动峰可归结为Ⅲ级酰胺的出峰；565，601 cm-1处的微弱峰主要是由磷酸盐的PO43-的O-P-O弯曲振动引起。5种骨红光结果提示其无机质成分主要为磷酸盐类与碳酸盐类。 2.3 五种松质骨的X射线衍射检测结果 5种松质骨的衍射峰大致相同，衍射峰宽大峰强弱，从2θ 30°-35°间的衍射主峰位置处未见明显分峰。用X射线衍射分析软件Jade 5.0对其进行物相分析，其中4种骨的主要衍射峰的位置与PDF#74-0565中羟基磷灰石的衍射峰(002)、(211)、(300)、(202)、(130)、(222)所在位置相对应，未见明显其他无机结晶相的衍射峰；老人松质骨在主衍射峰的(300)、(202)处相较其他骨可见分峰。5种骨衍射图提示松质骨中的无机结晶成分主要为羟基磷灰石，见图3。"

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