Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (38): 5650-5656.doi: 10.3969/j.issn.2095-4344.2016.38.004
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Received:
2016-07-05
Online:
2016-09-16
Published:
2016-09-16
Contact:
Ye Xiao-jian, M.D., Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200001, China
About author:
Fan Chun-quan, M.D., Attending physician, Orthopedics Center of PLA, the 175th Hospital of PLA/Dongnan Hospital of Xiamen University, Zhangzhou 363000, Fujian Province, China
Supported by:
CLC Number:
Fan Chun-quan, Ye Xiao-jian.
2.1.2 L929细胞与不同材料接触后的细胞黏附情况及形态学变化 在丝素蛋白/纳米羟基磷灰石复合材料浸提液组和阴性聚乙烯浸提液组细胞培养8 h后可以看见细胞形态基本正常,呈梭形或不规则三角形,细胞开始贴壁生长,随着时间延长,细胞数目增多,细胞突起伸展,出现分裂细胞。0.64%苯酚液阳性组在培养24 h时可见少量椭圆形细胞贴壁,大多数细胞悬浮。 培养3 d时可见丝素蛋白/纳米羟基磷灰石复合材料浸提液组(图2A)和聚乙烯浸提液组(图2B)细胞形态呈梭形或不规则三角形,细胞贴壁生长良好,排列密集规则,可见分裂细胞。培养3 d时在0.64%苯酚液组出现大部分细胞圆缩(图2C),死亡细胞明显增多,细胞基本不贴壁。因此通过细胞形态学观察,可以判定,丝素蛋白/纳米羟基磷灰石复合材料浸提液组和聚乙烯组浸提液无细胞毒性,0.64%苯酚液组具有重度细胞毒性。"
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