Effects of heavy load exercise and acupuncture treatment on tubulin in rat skeletal muscle

doi:10.3969/j.issn.2095-4344.2016.33.012

Abstract

Abstract:

BACKGROUND: Heavy load exercises can induce the ultrastructural changes in the skeletal muscle by the depolymerization and/or degradation of tubulin in the skeletal muscle, thereby lessening contraction activities of the skeletal muscle.
OBJECTIVE: To observe the effects of heavy load exercise and acupuncture on tubulin levels, and to analyze their roles and mechanisms in skeletal muscle injury and repair.
METHODS: 138 male Sprague-Dawley rats aged 8 weeks were randomly divided into acute exercise group (n=114) and long-term exercise group (n=24). The acute exercise group included four subgroups: sedentary group (n=6), exercise group (n=36), acupunctured group (n=36) and exercise plus acupuncture group (n=36). In the acute exercise experiment, rats were acupunctured after a medium-large intensity downhill running. Rat’s soleus samples were taken immediately, 6, 12, 24, 48 and 72 hours after exercise and/or acupuncture. In the long-term exercise experiment, rats underwent exercise and acupuncture for 3 weeks, and rat’s soleus samples were collected at 24 hours after the last training. Expressions of α-tubulin and microtubule-associated protein 4 (MAP4) in the soleus were detected by western blot assay.
RESULTS AND CONCLUSION: After acute exercise, expression of α-tubulin and MAP4 was up- or down-regulated transiently. After acute exercise combined with acupuncture, the protein expressions of α-tubulin and MAP4 changed slightly. However, the long-term exercise induced an increase in α-tubulin protein expression, while a significant decrease in MAP4 protein expression. It was worth noting that acupuncture treatment reduced long-term exercise-induced variations of MAP4 expression. These results suggest that the heavy load exercise can induce the depolymerization of tubulins in the skeletal muscle, and acupuncture may relieve this effect.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Sports Medicine, Cytoskeleton, Tubulin, Acupuncture

CLC Number:

R318

Liu Xiao-ran, Li Jun-ping, Wang Yun-hong, Wang Rui-yuan. Effects of heavy load exercise and acupuncture treatment on tubulin in rat skeletal muscle[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(33): 4949-4956.

Figures/Tables 2

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