Variation of stromal cell-derived factor-1 level in a limbal stem cell deficiency model

doi:10.3969/j.issn.2095-4344.2016.19.016

Abstract

Abstract:

BACKGROUND: How to promote homing of scarce limbal stem cells is the key to improving transplantation efficiency. And stromal cell-derived factor-1 plays an important role in the repair of retinal pigment epithelial cells injury.
OBJECTIVE: To observe changes of stromal cell-derived factor-1 levels in rat and mouse limbal stem cell deficiency models in order to confirm whether these changes will induce peripheral blood stem cells homing into the cornea.
METHODS: Eye models of limbal stem cell deficiency were prepared in rats and mice by cutting method, then peripheral blood samples were continuously drawn to detect the mass concentration of stromal cell-derived factor-1 by enzyme-linked immunosorbent assay within 1 month after modeling.
RESULTS AND CONCLUSION: In the rat peripheral blood, the mass concentration of stromal cell-derived factor-1 started to increase at 24 hours after modeling and firstly peaked at the 3rd day; then it tended to decrease and reached a second peak lower than the first one at the 25th day; subsequently, it returned to the normal level. In the meanwhile, the mass concentration of stromal cell-derived factor-1 in the mouse peripheral blood, increased at 12 hours after modeling, peaked at the 3rd day, subsequently decreased until another peak lower than the former one presented at the 20th day, and finally it returned to the normal level. In conclusion, the level of stromal cell-derived factor-1 in the peripheral blood is significantly associated with limbal stem cells deficiency, which may play an important role in corneal injury repair.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Tissue Engineering, Cornea

CLC Number:

R394.2

Zhang Wei, Huang Wen-zhi, Zhang Yu-ping. Variation of stromal cell-derived factor-1 level in a limbal stem cell deficiency model[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(19): 2844-2849.

Figures/Tables 1

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