Differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in vitro

doi:10.3969/j.issn.2095-4344.2016.19.005

Abstract

Abstract:

BACKGROUND: Previous studies have confirmed that as an exogenous origin of endometrial epithelial cells, bone marrow stem cells are involved in the functional reconstruction of the injured endometrium.
OBJECTIVE: To discuss whether bone marrow mesenchymal stem cells can differentiate into endometrial epithelial cells in vitro.
METHODS: Passage 2 bone marrow mesenchymal stem cells and endometrial stromal cells were collected and subjected to different treatments: bone marrow mesenchymal stem cells were cultured alone in the DMEM/F12 medium containing 2% fetal bovine serum or in the DMEM/F12 medium containing 2% fetal bovine serum, 10^-7 mol/L β-estradiol and 10 μg/L epidermal growth factor as groups 1 and 2, respectively; bone marrow mesenchymal stem cells co-cultured with endometrial stromal cells by Transwell chamber were cultured in the DMEM/F12 medium containing 2% fetal bovine serum or in the DMEM/F12 medium containing 2% fetal bovine serum, 10^-7 mol/L β-estradiol and 10 μg/L epidermal growth factor as groups 3 and 4, respectively. After 5 days culture, bone marrow mesenchymal stem cells at the bottom of the culture plate were collected to detect expressions of epithelial cell markers, such as CK7, CK18, CK1 and EMA by RT-PCR technology, and to determine keratin expression using immunofluorescence assay. Besides, expressions of these epithelial cell markers in the group 3 were detected using RT-PCR at 1, 3, 5 and 7 days of culture, respectively.
RESULTS AND CONCLUSION: mRNA expression of CK7 showed a significant successive rise in the groups 1, 2, 3, 4 and it was highest in the group 4. mRNA expressions of CK18 and CKl9 in the later three groups had no significant differences, but all significantly higher than those in the group 1. Compared with the groups 1 and 3, mRNA expression of EMA in the groups 2, 4 were significantly higher, but no significant differences existed between groups. Expression of keratin was strongly positive in the group 4, weakly positive in the group 3 and negative in the groups 1, 2, respectively. Furthermore, with the increase of culture time, mRNA expression of CK7 exhibited a constant increase, which was significantly higher at 5 and 7 days than at 1 and 3 days; but there were no significant differences in expressions of CKl9, CKl8 and EMA at different time points. These results show that bone marrow mesenchymal stem cells can differentiate into endometrial epithelial cells in vitro under certain conditions. Moreover, it can remarkably promote the endometrial differentiation under the combined effects of some exogenous and endogenous factors.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Endometrium, Epithelial Cells, Cell Differentiation, Coculture Techniques, Tissue Engineering

CLC Number:

R394.2

Qu Li-hong, Yu Jia-qun . Differentiation of bone marrow mesenchymal stem cells into endometrial epithelial cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(19): 2770-2776.

Figures/Tables 5

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