Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (5): 611-615.doi: 10.3969/j.issn.2095-4344.2016.05.001

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Establishing a large double-foot bird emu model of femoral head necrosis

Qu Chun-tao   

  1. Hongqiao Hospital, Tianjin 300131, China
  • Received:2015-12-21 Online:2016-01-29 Published:2016-01-29
  • About author:Qu Chun-tao, Master, Attending physician, Hongqiao Hospital, Tianjin 300131, China

Abstract:

BACKGROUND: There are many studies on the establishment of animal models of femoral head necrosis, but it is difficult to simulate an ideal animal model of femoral head necrosis, especially collapse models.
OBJECTIVE: To establish femoral head necrosis model of double-foot large bird emu so as to simulate femoral head necrosis in human.  
METHODS: A total of 30 adult emus were frozen in liquid nitrogen and received radiofrequency heating alternately to simulate femoral head necrosis models. After 3 cycles of freezing, local frozen in liquid nitrogen and method of radiofrequency heating was alternated for closed loop liquid nitrogen freezing injury and radiofrequency heating injury. At 6, 12 and 16 weeks after model establishment, gross observation, X-ray, histology examination and MRI were conducted. Effects of model induction of avascular necrosis of the femoral head were evaluated. 
RESULTS AND CONCLUSION: After liquid nitrogen cold-hot alternating method, emu at 12-18 weeks gradually suffered from femoral head necrosis signs and imaging findings. X-ray films, histological examination and MRI examination showed the avascular necrosis of femoral head was consistent with the pathological process of human femoral head necrosis, and the model had good repeatability. These results indicate that the use of liquid nitrogen cold-hot alternating method has successfully established the model of femoral head necrosis, with a good collapse rate, and can be used for mechanism research and treatment evaluation of osteonecrosis. 

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