Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (2): 197-201.doi: 10.3969/j.issn.2095-4344.2016.02.008

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Effect of uphill or downhill running training on articular cartilage degeneration and inflammatory responses of knee osteoarthritis rats

Wang Wen-sheng, Chen Wei, Shen Bao-lei, Xu Bo, Zhang Li, Jiang Zheng   

  1. Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • Received:2015-11-07 Online:2016-01-08 Published:2016-01-08
  • Contact: Jiang Zheng, M.D., Associate professor, Master’s supervisor, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • About author:Wang Wen-sheng, Studying for master’s degree, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China Chen Wei, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China Wang Wen-sheng and Chen Wei contributed equally to this work.
  • Supported by:

    A Project of Science and Technology Education Department of Fujian Province, No. JA12167

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Abstract:

BACKGROUND: Different training methods have different effects on primary knee osteoarthritis.
OBJECTIVE: To compare the effects of uphill or downhill running on articular cartilage degeneration and inflammatory responses of knee osteoarthritis rats.
 
METHODS: 108 female Sprague-Dawley rats were randomly divided into six groups: normal control group, ovariectomized group, uphill running group (run & up), downhill running group (run & down), uphill running model group (model & up), downhill running model group (model & down). The rats in the normal control group were raised routinely with no treatment; rats in the ovariectomized group were ovarientomized and raised in routine way; rats in the run & up group and run & down group were subjected to uphill running training on the slop +15° or downhill running training on the slop -15°; rats in the model & up group and model & down group were subjected to uphill running training on the slop +15°or downhill running training on the slop -15° after ovarientomized. Training programs were as follows: 28 m/min, 60 minutes per day, 6 days per week.
RESULTS AND CONCLUSION: Compared with the other five groups, in the model & up group, the Mankin’s score was significantly increased, indicating that articular cartilage degeneration occurred, and the peak of carboxy-terminal telopeptide of type II collagen contained in the urine had passed, at 4 weeks after modeling. At 6 weeks, the Mankin’s score of the model & down group was increased to reach the degeneration standard, but it was still lower than that of the model & up group; the expression levels of tumor necrosis factor-α and interleukin-1β in the synovial fluid were significantly higher in the model & up and model & down groups than the other four groups. At 8 weeks, the Mankin’s score and the expression levels of tumor necrosis factor-α and interleukin-1β were further increased in the model & up and model & down groups, especially in the model & up group. These results indicate that treadmill running exercise after ovariectomized can result in articular cartilage injury and local inflammatory responses; compared with the downhill running training, the uphill running training can cause cartilage injury earlier and more efficiently.