Cytokine-induced differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells under hydrostatic pressure in vitro

doi:10.3969/j.issn.2095-4344.2016.02.007

Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (2): 191-196.doi: 10.3969/j.issn.2095-4344.2016.02.007

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Cytokine-induced differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells under hydrostatic pressure in vitro

Chen Jiang¹, Jia Yu-song¹, Liu Gen-zhe², Sun Qi¹, Bai Wen-bo¹, Wang Li¹

¹First Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;²Department of Orthopaedics, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China

Received:2015-11-05 Online:2016-01-08 Published:2016-01-08
Contact: Jia Yu-song, M.D., Chief physician, Master’s supervisor, First Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
About author:Chen Jiang, M.D., Attending physician, First Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
Supported by:
Beijing Municipal Natural Science Foundation, No. 7142076; 2015 Fundamental Research Fund of Beijing University of Chinese Medicine (Middle and Young Teachers), No. 2015-JYB-JSMS084

Abstract

Abstract:

BACKGROUND: Differentiation of bone marrow mesenchymal stem cells is induced by integrated factors. In vitro interaction of cytokine complex and certain cell mechanical stimulation is carried out to further improve the efficiency of bone marrow mesenchymal stem cells differentiating into nucleus pulposus-like cells.

OBJECTIVE: To investigate the differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells induced by transforming growth factor-β1 and insulin-like growth factor-1 under hydrostatic pressure.

METHODS: Bone marrow mesenchymal stem cells from adult rats were separated, cultured and purified in vitro. Passage 3 cells were induced in vitro with transforming growth factor-β1 and insulin-like growth factor-1 under hydrostatic pressure (hydrostatic pressure group), with transforming growth factor-β1 and insulin-like growth factor-1 under normal pressure (drug group), or with normal culture medium under normal pressure (blank control group).

RESULTS AND CONCLUSION: At day 14 after culture, polygonal nucleus pulposus-like cells were observed in the hydrostatic pressure group, but irregular cells in the drug group. There was no obvious change in the blank control group. Levels of collagen type II and DNA were higher in the hydrostatic pressure group than the other two groups. These findings indicate that the combination of transforming growth factor-β1 and insulin-like growth factor-1 can successfully induce the differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells under hydrostatic pressure, and the differentiation efficiency is higher under hydrostatic pressure than under normal pressure.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Chen Jiang, Jia Yu-song, Liu Gen-zhe, Sun Qi, Bai Wen-bo, Wang Li. Cytokine-induced differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells under hydrostatic pressure in vitro[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(2): 191-196.

Figures/Tables 4

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Cytokine-induced differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells under hydrostatic pressure in vitro

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