Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7259-7262.doi: 10.3969/j.issn.2095-4344.2015.45.008

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Effect of astragalus polysaccharides on the neuronal differentiation of bone marrow mesenchymal stem cells

Wang Guan1, 2, Li Jing-ya3, Yang Sheng-ping1, 2, Wang Jian-min1, 2   

  1. 1The First Clinical Medicine College of Lanzhou University, Lanzhou 730000, Gansu Province, China; 2Department of Orthopedics, the First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China; 3Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China
  • Received:2015-08-11 Online:2015-11-05 Published:2015-11-05
  • Contact: Wang Jian-min, Professor, Master’s supervisor, Chief physician, the First Clinical Medicine College of Lanzhou University, Lanzhou 73000, Gansu Province, China; Department of Orthopedics, the First Hospital of Lanzhou University, Lanzhou 73000, Gansu Province, China
  • About author:Wang Guan, Studying for master’s degree, the First Clinical Medicine College of Lanzhou University, Lanzhou 73000, Gansu Province, China; Department of Orthopedics, the First Hospital of Lanzhou University, Lanzhou 73000, Gansu Province, China

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Abstract:

BACKGROUND: Active components of Astragalus have an antioxidant effect, which is considered to result in the neuron-like differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To investigate the effect of astragalus polysaccharides combined with basic fibroblast growth factor to induce the differentiation of bone marrow mesenchymal stem cells into nerve cells.
METHODS: After 24 hours of pretreatment with basic fibroblast growth factor, passage 3 human bone marrow mesenchymal stem cells were cultured with astragalus polysaccharides for 1-3 days (combined group). Blank control group and basic fibroblast growth factor group were set up. Expression of neuron-specific enolase and nestin was detected using western blot or immunocytochemical staining.
RESULTS AND CONCLUSION: The expression of neuron-specific enolase was higher in the combined group than the basic fibroblast growth factor group (P < 0.05). Expression of nestin was found in both basic fibroblast growth factor group and combined group, but the gray value was higher in the combined group than the basic fibroblast growth factor group (P < 0.05). These findings indicate that astragalus polysaccharides combined with basic fibroblast growth factor is better to induce the neuronal differentiation of bone marrow mesenchymal stem cells.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Fibroblast Growth Factor 2, Astragalus membranaceus, Cell Differentiation, Tissue Engineering