Fabrication and properties of nano-hydroxyapatite/poly(3-hydroxybutyrate) ultrafine-fibrous scaffolds for bone tissue engineering

doi:10.3969/j.issn.2095-4344.2015.25.010

Abstract

Abstract:

BACKGROUND: Poly(3-hydroxybutyrate) is approved as its excellent biocompatibility, biodegradability and piezoelectric properties, but there are also some deficiencies, such as high breakability and poor hydrophilicity.
METHODS: Poly(3-hydroxybutyrate) was mixed with different mass percentages of nanohydroxyapatite (0, 10%, 20% and 30%) to prepare new composite fibrous scaffolds through electrospinning process. The microstructure, group composition, crystalline phase distribution, thermal properties and surface wettability of the scaffolds were detected.
RESULTS AND CONCLUSION: Under the scanning electron microscope, with the increase of nano-hydroxyapatite content, more and more nano-hydroxyapatite particles were distributed evenly on the composite fiber surface; the fiber surface was basically covered with nano-hydroxyapatite particles at the content of 30%, and the roughness of the fiber surface also increased. Results from differential scanning calorimetry and
X-ray diffraction showed that the nano-hydroxyapatite reduced the crystallinity of poly(3-hydroxybutyrate) and the crystal tacticity, and this phenomenon became more evident with the increase of nano-hydroxyapatite content. Additionally, the higher the content of nano-hydroxyapatite content, the lower the contact angle and the higher the hydrophily. These findings indicate that the nano-hydroxyapatite/poly(3-hydroxybutyrate) ultrafine-fibrous scaffold using electrospinning technology can effectively improve the surface wettability and crystallinity of the material as well as the material hydrophily and brittleness, and the higher the content of nano-hydroxyapatite, the more obvious the effect.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Durapatite, Tissue Engineering, Contact Lenses, Hydrophilic

CLC Number:

R318

Guan Dong-hua, Lin Ying-he, Huang Jian-sheng, Chen Zhi-qing . Fabrication and properties of nano-hydroxyapatite/poly(3-hydroxybutyrate) ultrafine-fibrous scaffolds for bone tissue engineering [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(25): 3983-3989.

Figures/Tables 5

References

[1] Langer R,Tirrell DA.Designing materials for biology and medicine. Nature.2004; 428(6982):487-492.

[2] Navarro M,Ginebra MP,Planell JA,et al.Development and cell response of a new biodegradable composite scaffold for guided bone regeneration.J Mater Sci Mater Med.2004; 15(4):419-422.

[3] Kim HW,Kim HE,Salih V.Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin-hydroxyapatite for tissue engineering scaffolds. Biomaterials.2005;26(25): 5221-5230.

[4] Sinha A,Das C,Sharma BK,et al.Poly (vinyl alcohol)- hydroxyapatite biomimetic scaffold for tissue regeneration. Mater Sci Eng C Biomimetic Supramol Sys.2007;27(1):70-74.

[5] Zhou YF,Hutmacher DW,Varawan SL,et al.In vitro bone engineering based on polycaprolactone and polycaprolactone-tricalcium phosphate composites.Polym Int.2007;56(3):333-342.

[6] Zhang RY,Ma PX.Poly(alpha-hydroxyl acids) hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology.J Biomed Mater Res.1999; 44(4):446-455.

[7] Slater SC,Gallaher T,Dennis D,et al.Polyhydroxybutyrate. Natural biocompatible and biodegradable polyesters produced by bacteria.J Bacteria.1988;170(10):4431-4436.

[8] Doyle C,Tanner ET,Bonfield W.In vitro and in vivo evaluation of polyhydroxybutyrate to and of polyhydroxybutyrate reinforced with hydroxyapatite. Biomaterials.1991;12(11): 841-847.

[9] Boeree NR,Dove J,Cooper JJ,et al.Development of a degradable composite for orthopaedic use: mechanical evaluation of a hydroxyapatite-polyhydroxybuty- rate composite material.J Biomaterials.1993;14(10):793-796.

[10] Barham PJ,Kelley A,Otnn EL,et al.Crystallization and morphology a bacterial thermoplastic:poly-3-hydroxybutyrate. J Mater Sci.1981;19:2781-2794.

[11] Ma ZW,Kotaki M,Yong T,et al.Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering.Biomaterials.2005;26(15):2527-2536.

[12] Taylor GI.Electrically driven jets. Proc R Soc London Ser A.1969;313:453-475.

[13] Fong H,Reneker DH.Electrospinning and formation of nano-fibers.In:Salem DR, editor. Structure formation in polymeric fibers. Munich: Hanser,2001:225-246.

[14] Huang ZM,Zhang YZ,Kotaki M.A review on polymer nanofibers by electrospinning and their applications in nanocomposites.ComposSci Technol.2003;63:2223-2253.

[15] Deitzel JM,Kleinmeyer J,Harris DT.The effect of processing variables on the morphology of electrospun nanofibers and textiles.Polymer.2001;42:261-272.

[16] Demir MM,Yilgor I,Yilgor E,et al.Electrospinning of polyurethane fibers. Polymer.2002;43:3303-3309.

[17] Casper CL,Stephens JS,Chase D,et al.Using humidity and molecular weight to control the surface morphology of electrospun fibers.Abstr Pap Am Chems.2003;226: U430- U430, 496-POLY Part 2.

[18] Zong X,Kim K,Fang D,et al.Structure and process relationship of electrospun bioabsorbable nanofiber membranes. Polymer. 2002; 43(16):4403-4412.

[19] Liu W,Wu ZQ,Reneker DH.Structure and morphology of poly (meta-phenylene isophthalamide) nanofibers produced by electrospinning. Abstr Pap Am Chem Soc.2000;220: U260- U260.

[20] Deitzel JM,Kleinmeyer JD,Hirvonen JK.Controlled deposition of electrospun poly(ethylene oxide) fibers.Polymer.2001;42: 8163-8170.

[21] 方壮熙,张璐,韩涛,等.PHBV电纺纤维结构与形态的研究[J].高分子学报,2004,48(4):500-505.

[22] Barham PJ,Kelley A,Otnn EL,et al.Crystallization and morphology a bacterial thermoplastic: poly-3-hydroxybntyrate. J Mater Sci.1981;19:2781.