Neural stem cells: in vitro culture, identification and differentiated phenotype

doi:10.3969/j.issn.2095-4344.2015.19.018

Abstract

Abstract:

BACKGROUND: Neural stem cells can repair the damaged central nervous system structure and function, which have broad application prospects. It can be realized by studies on in vitro culture, identification and differentiation phenotype of neural stem cells.
OBJECTIVE: To observe the biological characteristics and differentiated phenotypes of neural stem cells under induction in vitro.
METHODS: Neural stem cells were extracted from the hippocampus and olfactory bulb of newborn mice. After three generations, neural stem cells were labeled with 5-bromo-2-deoxyuridine (BrdU) and identified by immunofluorescence staining of BrdU, nestin and Hochest33258. The differentiation of neural stem cells was induced in vitro and identified by immunofluorescent staining of BrdU, β-tubulinⅢ, glial fibrillary acidic protein and Hochest33258.
RESULTS AND CONCLUSION: After passage, neural stem cells from the hippocampus and olfactory bulb of newborn mice could be aggregated into neurospheres that were positive for nestin and BrdU. Under induced differentiation in vitro, neural stem cells gradually turned into daughter cells which positively expressed β-tubulin III or glial fibrillary acidic protein. These findings suggest that neural stem cells have strong self-renewal capacity and have the tendency to form neurospheres during culture in vitro; under in vitro induction, they can differentiate
into neurons and astrocytes through asymmetric cell proliferation and differentiation.

CLC Number:

R394.2

Liu Ji-xing, Hou Bo-ru, Yang Wen-zhen, Ma Jun-ning, Yan Gui-zhong, Chen Si-hua, Yin Li-shan, . Neural stem cells: in vitro culture, identification and differentiated phenotype[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3054-3060.

Figures/Tables 4

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