Nano-hydroxyapatite/silk fibroin bone substitute for repairing bone defects

doi:10.3969/j.issn.2095-4344.2015.08.008

Abstract

Abstract:

BACKGROUND: To obtain a more ideal bone substitute material, nano-hydroxyapatite/silk fibroin composite material has been prepared in the preliminary experiments.

OBJECTIVE: To investigate the effect of nano-hydroxyapatite/silk fibroin composite material for repair of bone defects.

METHODS: Thirty healthy adult rabbits were randomly divided into two groups, and unilateral middle and distal femoral bone defect models were prepared. The experimental group was implanted with nano-hydroxyapatite/silk fibroin composite material, and the control group was implanted with pure hydroxyapatite. At 4, 8, 12 weeks after surgery, the femur was taken from each rabbit for gross, X-ray, biomechanical and histological examinations.

RESULTS AND CONCLUSION: In both two groups, biomechanical strength was gradually increased with time, but the biomechanical strength was higher in the experimental group than the control group at different time points (P < 0.05). At 12 weeks after surgery, in the experimental group, the bone graft had the smooth surface and exhibited normal color with no difference from the surrounding bone, the artificial bone material was completely absorbed and degraded, and bone defects were completely healed; in the control group, the bone graft had no smooth surface on which the bone cortex was continuous and showed unclear boundary with the surrounding bone tissues, the artificial bone materials were incompletely degraded, bone defects were connected by the calluses, the artificial bone materials were closely in conjunction with the surrounding bone tissues, and bone defects were partially repaired. These findings indicate that the nano-hydroxyapatite/silk fibroin composite artificial material can promote bone defect repair and exhibit stronger osteogenic ability.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Hydroxyapatites, Silk, Compomers

CLC Number:

R318

Sun Qing-zhi . Nano-hydroxyapatite/silk fibroin bone substitute for repairing bone defects[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(8): 1190-1194.

Figures/Tables 2

2.1 一般情况 30只家兔术后饮食及活动正常，无死亡，无感染发生，均进入结果分析。 2.2 大体观察结果实验组：植入后4周，骨缺损植入区表面有结缔组织生成，植骨区与周围骨组织界限模糊，伴有大量骨痂形成；植入后8周，植骨区与周围骨组织无明显界限，但骨表面凸凹不平，骨结合紧密；植入后12周，植骨区表面连续且光滑，色泽正常，与周围骨质无明显区别。对照组：植入后4周，骨缺损植入区表面少量结缔组织生成，植骨区与周围骨组织界限清晰，伴有少量骨痂形成；植入后8周，植骨区与周围骨组织界限模糊，仍可见凸凹处植入的人工骨材料，骨结合不紧密；植入后12周，植骨区表面不光滑，表面骨皮质连续，与周围骨质界限不清。 2.3 X射线检查结果实验组：植入4周时，植入材料与正常骨组织界面见一狭窄线状阴影，骨痂形成；植入8周时，人工骨材料与骨组织基本骨性结合，界限模糊不清，成骨由两端向中心生长；植入12周时，人工骨材料被新生骨质替代，骨缺损完全被修复(图1A)。对照组：植入4周时，植入材料与正常骨组织交界处可见狭窄线状阴影，骨痂形成，与实验组无明显差异；植入8，12周时，人工骨材料降解不完全，骨缺损处由骨痂连接，骨缺损部分修复(图1B)。根据Lane-Sandhu X射线评分，两组植入8，12周的骨组织修复比较差异有显著性意义(P < 0.05)，见表1。 2.4 组织形态学检查结果实验组：植入4周时，树支样成纤维细胞渗入到人工骨材料中并有类骨质样物生成，人骨材料少量降解；8周时，新骨生成较4周时明显增多，伴有大量板层骨质生成，人工骨材料被包围成小的颗粒样，进一步降解吸收；12周时，人工骨材料完全降解吸收，骨缺损区已完全骨愈合(图2A)。对照组：植入4周时，材料与骨之间有间隙，少许材料降解；8周时，人工骨材料中间有骨质生成，人工骨材料仍少许降解；12周时，人工骨材料还有许多未降解，材料与骨质结合较紧密，骨缺损部分修复(图2B)。根据Lane-Sandhu组织学评分，两组材料植入4，8，12周的骨组织修复比较差异有显著性意义(P < 0.05)，见表2。随着时间骨组织修复时间的延长，实验组逐渐形成成熟的哈佛氏系统，骨小梁排列整齐；对照组还有部分未降解的人工材料，骨松质生长活跃，骨皮质形成不连续。 2.5 扫描电镜观察结果实验组：植入4周时，周围正常骨质与材料之间有明显间隙；植入8周时，人工骨材料部分降解，人工骨材料与周围骨组织结合紧密；植入12周时，人工骨材料完全降解，骨缺损区被新生的骨质替代，骨缺损完全被修复(图3A)。对照组：植入4周时，周围正常骨质与材料之间有明显间隙；植入8周时，人工骨材料与周围骨组织间隙变小，部分结合紧密；植入12周时，人工骨材料仍大部分未降解，骨缺损部分修复(图3B)。 2.6 生物力学检测结果三点弯曲强度数据以t 检验统计学分析显示实验组优于对照组(P < 0.05)；随着时间的增长，两组组内生物力学强度逐渐越大，见表3。"

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