Bone marrow mesenchymal stem cells are induced directly to differentiate into chondrocytes in osteoarthritis patients

doi:10.3969/j.issn.2095-4344.2015.06.003

Abstract

Abstract:

BACKGROUND: In recent years, many reports have focused on inflammatory cytokines, growth factors and mechanical loads affecting the cartilage and subchondral regeneration, but there is a lack of comprehensive understanding about the mechanism of osteoarthritis.
OBJECTIVE: To explore the correlation between function status of bone marrow mesenchymal stem cells and disease progression in patients with osteoarthritis.
METHODS: Femoral bone marrow was extracted from patients with femoral neck fractures (control group), mild (mild group) and severe (severe group) osteoarthritis to isolate and culture bone marrow mesenchymal stem cells. Cell counting kit-8 was used to detect the proliferative ability of bone marrow mesenchymal stem cells from
different patient groups, and passage 3 bone marrow mesenchymal stem cells were subject to 2-week chondrogenic induction followed by toluidine blue staining.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells were isolated and cultured from the femoral bone marrow of different groups. The proliferative ability of cells in the control group was significantly higher than that in the mild and severe groups. After chondrogenic induction, bone marrow mesenchymal stem cells varied obviously in the morphology that was from fusiform to qusi-circular or polygon, the percentage of nucleoplasm became smaller, and cells were positive for toluidine blue staining. The number of chondrocytes generated in the severe group was less than that in the control group, but there was no great difference in cell morphology. These findings indicate that the occurrence of osteoarthritis is negatively correlated with the functional status of autologous bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Osteoarthritis, Cell Proliferation

CLC Number:

R394.2

Guo Hong-liang, Tie Xiao-jia, Han Ya-jun, Xu Chao, Wang Zhi-zhou, Wang Xin, Wang Jun-feng, Guan Zhen-peng, Bi Xiao-juan, Yilihamu Tuoheti. Bone marrow mesenchymal stem cells are induced directly to differentiate into chondrocytes in osteoarthritis patients[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(6): 832-836.

Figures/Tables 3

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