Role of hypoxia-inducible factor 1 alpha and inducible nitric oxide synthase in neural stem cells during hypoxia/reoxygenation injury

doi:10.3969/j.issn.2095-4344.2015.06.002

Abstract

Abstract:

BACKGROUND: Organism in the hypoxic state can produce hypoxia-inducible factor, but nitric oxide generated from inducible nitric oxide synthase can inhibit the activity of hypoxia-inducible factor 1α.
OBJECTIVE: To discuss the relationship of hypoxia-inducible factor 1α and inducible nitric oxide synthase with hypoxia/reoxygenation injury in neural stem cells.
METHODS: Under sterile conditions, Wistar rats born within 24 hours were sacrificed to separate the rat hippocampus that was used to prepare a cell suspension of brain tissue. After culture and passage, neural stem cells were divided into normoxia, hypoxia and hypoxia/reoxygenation groups. In the latter two groups, 150 μmol/L cobalt chloride solution was used to prepare hypoxia models, and in the hypoxia/reoxygenation group, the cells were reoxygenated after 4-hour hypoxia.
RESULTS AND CONCLUSION: Under hypoxic conditions, a significant increase in mRNA expressions of hypoxia-inducible factor 1α and inducible nitric oxide synthase as well as the number of apoptotic neural stem cells. Compared with the hypoxia group, the number of apoptotic neural stem cells was higher in the hypoxia/reoxygenation group, but the mRNA expressions of hypoxia-inducible factor 1α and inducible nitric oxide synthase were lower. These findings indicate that these two factors are involved in the hypoxia/reoxygenation injury of neural stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Nitric Oxide Synthase, Anoxia, Neural Stem Cells, Cytokines

CLC Number:

R394.2

Li Ying. Role of hypoxia-inducible factor 1 alpha and inducible nitric oxide synthase in neural stem cells during hypoxia/reoxygenation injury[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(6): 826-831.

Figures/Tables 3

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