Repairing rabbit’s radial bone defects using injectable nano-hydroxyapatite composite scaffold co-cultured with bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.03.09

Abstract

Abstract:

BACKGROUND: Nano-hydroxyapatite/chitosan/calcium sulphate hemihydrate (nHA/CS/CSH) composite scaffold constructed in preliminary experiments has good physical and chemical properties.

OBJECTIVE: To explore the effects of injectable nHA/CS/CSH scaffold co-cultured with bone marrow mesenchymal stem cells (nHA/CS/CSH/BMSCs) for repairing rabbit’s radial bone defects.

METHODS: Bone defects with the length of 10 mm were created on the middle of the bilateral radial bones of 18 New Zealand white rabbits. The defects were filled with the nHA/CS/CSH/BMSCs composite as treatment group, while the others were filled with HA as controls. The ability of repairing bone defects was evaluated by gross, X-ray and histopathological observation at 4, 8, 12 weeks after implantation.

RESULTS AND CONCLUSION: At the end of 12 weeks after implantation, in the treatment group, bone defects were completely repaired, the radial bone showed normal morphology, the bone marrow cavity was recanalized a ittle from both ends toward the middle, images in the defect area had no difference from normal bone tissues, a large amount of new lamellar bone tissues were visible, the Harvard’s system formed, the original defects were filled with newborn lamellar bone tissues, bone tissues were interconnected, and the bone marrow cavities was interlinked; in the control group, bone defect was repaired partly, the bone mineral density was lower than that of the normal bone tissue, the bone marrow cavities were recanalized partially, and a few of new lamellar bone tissues, accompanied with connective tissues growing into the defects. The nHA/CS/CSH/BMSCs is effective for the repair of bone defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Tissue Engineering, Hydroxyapatites, Chitosan

CLC Number:

R318

Xue Zhen, Niu Li-yuan, An Gang, Guo Ya-shan, Lv Song-cen. Repairing rabbit’s radial bone defects using injectable nano-hydroxyapatite composite scaffold co-cultured with bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(3): 378-383.

Figures/Tables 4

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