Biological characters of mesenchymal stem cells separated from different components of human placenta

doi:10.3969/j.issn.2095-4344.2014.19.021

Abstract

Abstract:

BACKGROUND: Human placenta is a stable source for mesenchymal stem cells, which is becoming a cell source in the regenerative medicine that attracts widespread attentions.
OBJECTIVE: To compare the biological characters of mesenchymal stem cells that separated from different components of human placenta (human chorion and villous trophoblast).
METHODS: The amniotic membrane of placenta surface was detached surgically. Human chorion and villous trophoblast in the fetal side was cut into pieces. After digestion with PBS containing collagenase II, mononuclear cells were separated and cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum in 37 ℃ and 5% CO2, 95% saturated humidity, after 48 hours the full amount in liquid, dislodge suspension cells. Forty-eight hours later, the medium was changed completed, and non-adherent cells were removed. When cell fusion reached about 90%, trypsin digestion was employed for cell passage. Biological characters of mesenchymal stem cells separated from different components of human placenta were compared through observation of total number of primary cells, cell morphology, and surface markers expression (CD90, CD73 and CD105).
RESULTS AND CONCLUSION: The flow cytometric analysis revealed that the cells separated from the human chorion and villous trophoblast were over 90% strongly positive for CD90, CD73, CD105. These two sources of cells showed typical fibroblast morphology, suggesting that the cells have the characteristics of mesenchymal stem cells. Under the same enzyme digestion time, enzyme concentration, and shaking speed, more cells are visible from the chorion, and the subsequent culture is easier to harvest cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, placenta, mesenchymal stem cells, chorion, collagenases

CLC Number:

R394.2

Hong Yan, Huo Si-wei, Lu Yao, Zhang Yi. Biological characters of mesenchymal stem cells separated from different components of human placenta[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(19): 3082-3087.

Figures/Tables 3

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