Growth factors promote the therapeutical effects of autologous bone marrow mesenchymal stem cell transplantation on acute myocardial infarction

doi:10.3969/j.issn.2095-4344.2014.19.011

Abstract

Abstract:

BACKGROUND: The growth factor is a potent mobilization agent for stem cells, which can increase adhesion and proliferation of injected cells, induce stem cells to migrate to the infarct zone, proliferate, differentiate, as well as participate in myocardiac repair.
OBJECTIVE: To investigate the effect of hepatocyte growth factor (HGF) and insulin-like growth factor (IGF) on transplantation of bone marrow mesenchymal stem cells (BMSCs) after acute myocardial infarction.
METHODS: Primary rabbit BMSCs were cultured in vitro and labeled by red fluorescence dye CM-Dil for transplantation. After the mid third of left anterior descending was ligated, model rabbits were grouped into four groups: control group, BMSCs group, HGF+IGF group, and HGF+IGF+BMSCs group (n=6 in each group). Different interventional agents were injected into the myocardium at four sites within the ischemic region. Masson trichrome staining was performed to determine viable myocardium, and immunofluorescence staining was used to identify BMSCs differentiation. The cardiac function was assessed with Doppler echocardiography.
RESULTS AND CONCLUSION: Four weeks after treatment, CM-Dil/cTNT+ cells significantly increased in the HGF+IGF+BMSCs group, compared with BMSCs group. Consequently, viable myocardial tissues significantly increased, left ventricular ejection fraction was significantly improved, and left ventricular end-diatolic volume significantly decreased in the HGF+IGF+BMSCs group, relative to the other three groups. Combination of HGF and IGF that promotes differentiation of transplanted autologous BMSCs into cardiomyocytes, thus increasing viable myocardium, improving left ventricular function, and inhibiting left ventricular remodeling, may be a new method for the cell treatment of acute myocardial infarction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, bone marrow, mesenchymal stem cells, myocardial infarction, myocytes, cardiac, hepatocyte growth factor

CLC Number:

R394.2

Wen Ti, Qi Xun. Growth factors promote the therapeutical effects of autologous bone marrow mesenchymal stem cell transplantation on acute myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(19): 3017-3022.

Figures/Tables 3

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