Human umbilical cord mesenchymal stem cells: isolation, identification and multi-directional differentiation

doi:10.3969/j.issn.2095-4344.2014.19.010

Abstract

Abstract:

BACKGROUND: The umbilical cord mesenchymal stem cells can be obtained conveniently, noninvasively, without ethical restrictions, which are more original that stem cells from other sources and have little immunogenicity. Therefore, umbilical cord mesenchymal stem cells are a kind of ideal seed cells with promising application prospects.
OBJECTIVE: To induce the differentiation of mesenchymal stem cells derived from human umbilical cord into adipocytes and osteoblasts.
METHODS: The umbilical cord mesenchymal stem cells were isolated by tissue explants method. The morphology, proliferation and immunophenotype of the 3rd passage cells were analyzed, and then cells were induced to adipocytes and osteoblasts in vitro. The expressions of CD90, CD105, CD34 and CD15 were detected by flow cytometry.
RESULTS AND CONCLUSION: The umbilical cord mesenchymal stem cells isolated by tissue explants method could be cultured and proliferated in vitro. Flow cytometry analysis revealed that the cells were strongly positive for CD90 and CD105, but negative for CD34 and CD45. The umbilical cord mesenchymal stem cells could be induced to adipocytes and osteoblasts in vitro. These findings indicate that mesenchymal stem cells can be successfully obtained from human umbilical cord by tissue explants method and have the potential of multi-directional differentiation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: umbilical cord, mesenchymal stem cells, cell culture techniques, cell differentiation, adipogenesis

CLC Number:

R394.2

Li Mao, Huang Wen . Human umbilical cord mesenchymal stem cells: isolation, identification and multi-directional differentiation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(19): 3012-3016.

Figures/Tables 3

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