Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (19): 2999-3004.doi: 10.3969/j.issn.2095-4344.2014.19.008

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Effect and mechanism of mesenchymal stem cells on the aging kidney

Li Yan-ju1, Liu Yang2, Wang Ji-shi1, Meng Wei3, Huang Yi1   

  1. 1Department of Hematology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
    2Department of Life Science and Biomedical Engineering, Beijing University of Technology, Beijing 100022, China
    3Department of Genetic Engineering, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Revised:2014-04-17 Online:2014-05-07 Published:2014-05-07
  • Contact: Li Yan-ju, Department of Hematology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
  • About author:Li Yan-ju, M.D., Associate chief physician, Department of Hematology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
  • Supported by:

    the Science and Technology Plan Fund of Guizhou Province, No. SY[2010]3075; the Grant from the Health Bureau of Guizhou Province, No. gzwkj2011-1-023

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can differentiate into renal tubular epithelial cells. It is speculated that mesenchymal stem cells may have a therapeutic role in chronic renal dysfunction.
OBJECTIVE: To study the effect of bone marrow mesenchymal stem cells on the aging kidney of rats and its possible mechanism.
METHODS: Aging models were established in rats. Rat bone marrow mesechymal stem cells cultured in vitro were labeled by 5,6-carboxyfluorescein diacetate, succinimidyl ester, and then injected into the rats via the tail vein. Under a fluorescence microscope, the cell homing was observed. The contents of urea nitrogen and creatinine in serum of rats were tested by automatic biochemical analyzer. The overlaying of blue fluorescent 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride and the specific protein of red fluorescent tubular epithelial cells (keratin protein) was detected by indirect immunofluorescence and computer image stacking technique.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells could arrive the kidney of rats. Compared with the model group, the contents of urea nitrogen and creatinine in serum were decreased in the treated group (P < 0.05). The treated group showed a small amount of double positive cells. These findings indicate that rat bone marrow mesenchymal stem cells may differentiate into renal tubular epithelial cells, which may be one of mechanisms that improve the aging kidney of rats.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


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Key words: renal insufficiency, mesenchymal stem cells, kidney tubules, cell differentiation

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