Human umbilical cord mesenchymal stem cells are induced in vitro to differentiate into fibroblasts

doi:10.3969/j.issn.2095-4344.2014.10.012

Abstract

Abstract:

BACKGROUND: The umbilical cord mesenchymal stem cells possess multipotent differentiation capacity, but less research focus on its differentiation into fibroblasts.
OBJECTIVE: To investigate the capacity of human umbilical cord mesenchymal stem cells differentiating into fibroblasts.
METHODS: Using adherent method, human umbilical cord mesenchymal stem cells were isolated, and flow cytometric analysis of the surface antigen was performed. Passage 3 cells were selected for osteogenic and adipogenic differentiation, and cells differentiated into fibroblasts under the induction of basic fibroblast growth factor.
RESULTS AND CONCLUSION: Adherent stem cells were stably isolated from the umbilical cord. Human umbilical cord mesenchymal stem cells lowly expressed CD31, CD45, CD40, HLA-DR, but strongly expressed CD29, CD90, CD44, CD105. Oil red O staining showed red droplets were full of the cytoplasm after adipogenic induction; alizarin red staining showed red calcium nodules after osteogenic induction. After induced by basic fibroblast growth factor, the type I collagen expression was significantly higher than that in the control group. These findings indicate that the adherent human umbilical cord mesenchymal stem cells are reliably isolated with high purity; basic fibroblast growth factor can induce differentiation of umbilical cord mesenchymal stem cells into fibroblasts.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, umbilical cord, fibroblasts

CLC Number:

R394.2

Yang Yi, Luo Xin, Jiang Xue-feng, Shuai Han-lin, Song Hong, Zhang Jing-li, Lan Jian-fa. Human umbilical cord mesenchymal stem cells are induced in vitro to differentiate into fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1554-1559.

Figures/Tables 4

References

[1]Zickri MB, El Aziz DH, Metwally HG. Histological experimental study on the effect of stem cell therapy on adriamycin induced chemobrain. Int J Stem Cells. 2013;6(2):104-112.

[2]Nirmal RS, Nair PD. Significance of soluble growth factors in the chondrogenic response of humanumbilical cord matrix stemcells in a porous three dimensional scaffold. Eur Cell Mater.2013;26:234-251.

[3]Ruan ZB, Zhu L, Yin YG, et al.The mechanism underlying the differentiation of human umbilical cord-derived mesenchymal stem cells into myocardial cells induced by 5-azacytidine. Indian J Med Sci. 2010;64(9):402-407.

[4]Chen X, Zhang F, He X,et al.Chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells in type I collagen-hydrogel for cartilage engineering. Injury.2013; 44(4):540-549.

[5]Xu Y, Meng H, Li C,et al.Umbilical cord-derived mesenchymal stem cells isolated by a novel explantation technique can differentiate into functional endothelial cells and promote revascularization. Stem Cells Dev. 2010; 19(10):1511-1522.

[6]Jackson L, Jones DR, Scotting P, et al.Adult mesenchymal stem cells: differentiation potential and therapeutic applications. J Postqrad Med. 2007; 53(2):121-127.

[7]Passier R, van-Laake LW, Mummery CL.Stem-cell-based therapy and lessons from theheart. Nature.2008;453(7193): 322-329.

[8]Zhang Y, Cai W, Huang Q, et al.Mesenchymal Stem Cells Alleviate Bacteria-Induced Liver Injury in Mice by Inducing Regulatory Dendritic Cells.Hepatology. 2014;59(2):671-682.

[9]Ohmura Y, Tanemura M, Kawaguchi N, et al.Combined transplantation of pancreatic islets and adipose tissue-derived Stem Cells enhances the survival and insulin function of islet grafts in diabetic mice. Transplantation.2010;90(12): 1366-1373.

[10]Sacco A, Mourkioti F, Tran R, et al. Short Telomeres and Stem Cell Exhaustion Model Duchenne Muscular Dystrophy in mdx/mTR Mice.Cell. 2010;143(7):1059-1071.

[11]Jameel Mohammad Nuruqadr, Zhang Jianyi.Stem cell threapy for Ischemic Heart Disease. Antioxidants and Redox Signaling. 2010;13(12):1879-1897.

[12]徐丽南,姚志成,林楠,等.人脐血间充质干细胞对T淋巴细胞旁分泌的免疫调节作用[J].中国组织工程研究与临床康复,2011, 15(40):7463-7466.

[13]刘伟,刘萌,祝劲松,等.人骨髓间充质干细胞的体外培养、鉴定及成骨分化[J].中国组织工程研究,2012,16(14):2515-2519.

[14]Camassola M, de Macedo Braqa LM, Chaqastelles PC, et al. Methodology, biology and clinical applications of human mesenchymal stem cells. Methods Mol Biol. 2012;879:491- 504.

[15]潘指挥,王丽,刘瑞风,等.皮肤间充质干细胞的原代培养[J].中国皮肤性病学杂志,2012,26(2):114-117.

[16]Friedenstein AJ, Gorskaja JF, Kulagina NN. Fibroblast precursors in normal and irradiatedmouse hematopoietic organs. Exp Hematol.1976;4(5):267-274.

[17]Caplan AI. Mesenchymal stem cells. J Orthop Res.1991;9 (5):641-650.

[18]罗新,高绿芬,陈翠平. 17雌二醇对骨髓间充质干细胞增殖及成肌分化作用的研究[J].中国妇产科临床杂志.2010,11(5):369-373.

[19]Pittenger MF, Maekay AM, Beck SC,et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-147.

[20]Sobolewski K,Bańkowski E,Chyczewski L, et al. Collagen andglycosaminoglycans of Wharton’s jelly. Biol Neonate. 1997;71(1):11-21.

[21]任瑞芳,任琛琛,赵冰,等.体外分离共培养诱导大鼠骨髓间充质干细胞定向分化为子宫韧带成纤维细胞的研究[J].实用妇产科杂志,2011,27(2):110-113.

[22]代媛媛,原工杰,余桂戎,等.间接共培养诱导大鼠骨髓间充质干细胞向牙周膜成纤维样细胞分化[J].临床口腔医学杂志,2010, 26(5): 275-277.

[23]Vanjak-Novakovic G, Altman G, Horan R, et al. Tissue engineering of ligaments. Annu Rev Biomed Enq. 2004; 6:131-156.

[24]罗新.女性盆底解剖结构的新概念[J].中国实用妇科与产科杂志, 2006,22(1):78-80.

[25]张艳君,梅虎,蒋稼欢,等.共培养下后交叉韧带成纤维细胞中赖氨酰氧化酶的基因表达[J].中国组织工程研究,2013,17(20): 3692-3698.

[26]Dominici M,Le Blanc K, Mueller I, et al.Minimal criteria for definingmultipotent mesenchymal stromal cells. The International Societyfor Cellular Therapy position statement. Cytotherapy.2006;8 (4) :315-317.

[27]Burdick JA, Vunjak-Novakovic G.Engineered Microenvironments for Controlled Stem Cell Differentiation. Tissue Eng Part A.2009;15(2):205-219.

[28]Brafman David A. Constructing stem cell microenvironments using bioengineering approaches. Physiological Genomics. 2013;45(23):1123-1135.

[29]何绍清,罗振宇,刘秋英,等.人脐带间充质干细胞分离培养及向脂肪和成骨细胞的分化[J].中国组织工程研究与临床康复,2010, 14(14):2492-2495.

[30]Lee CH, Moioli EK, Mao JJ. Fibroblastic differentiation of human mesenchymal stem cells using connective tissue growthfactor.Conf Proc IEEE Eng Med Biol Soc.2006; 1: 775-778.

[31]Liu HF, Fan HB, Wang Y. The interaction between a combinedknitted silk scaffold and microporous silk sponge with human mesenchymal stem cells for ligament tissue engineering. Biomaterials.2008;29(6):662-674.

[32]杜尧,李晓声,张娜娟.大鼠骨髓间充质干细胞及大隐静脉来源细胞向组织工程化韧带种子细胞诱导的比较[J].中国组织工程研究与临床康复,2010,14(19):3427-3430.

[33]孙智晶,郎景和,朱兰,等.胶原状态与压力性尿失禁的病因学研究[J].中国妇产科临床杂志,2004,5(1):6-8.

[34]Liapis A ,Bakas P ,Pafiti A, et al. Changes in the quantity of collagen type I in women with genuine stress incontinence. Urological Research. 2000;28(5):323-326.

[35]罗新,姚润斯,宋泓,等.人脐带间充质干细胞移植治疗大鼠压力性尿失禁的研究[J].中华妇产科杂志,2013,48(8):1-5.

[36]Kyung CS, Sun-Ouck K, Soo YJ,et al.Effects of Mesenchymal Stem Cells on StressIncontinence in a Rat Model. Korean J Urol.2008;49:432-438.

[37]Wu G,Song Y,Zheng X,et al.Adipose-derived stromal cell transplantation for treatment ofstress urinary incontinence. Tissue Cell.2011;43(4):246-253.

[38]徐惠成,王延洲,王丹,等.肌源性干细胞治疗压力性尿失禁的实验研究[J].第三军医大学学报,2007,29(2):104.

[39]石海燕,蓝建发,罗新.间充质干细胞治疗压力性尿失禁从动物实验到临床应用的转化.中华腔镜泌尿外科杂志:电子版,2013, 7(5):1-4.