Human amniotic epithelial cell transplantation for repair of brachial plexus injury in rabbits

doi:10.3969/j.issn.2095-4344.2013.53.010

Abstract

Abstract:

BACKGROUND: A great deal of attention has been given to stem cell transplantation for repair of spinal cord injury, while there are few studies concerning stem cell transplantation for repair of peripheral nerve injuries, especially transplantation of human amniotic epithelial cells used to repair peripheral nerve injuries.
OBJECTIVE: To discuss the effects of human amniotic epithelial cells in brachial plexus nerve repairing.
METHODS: We established rabbit models of brachial plexus injuries and extracted human amniotic epithelial cells for expanding culture followed by ultrastructural observation of human amniotic membrane tissue and amniotic epithelial cells under a scanning electron microscope. Then, human amniotic epithelial cells were transfected with green fluorescent protein markers and injected into the injury site. Hematoxylin-eosin staining and fluorescence microscope were employed to observe the effects of human amniotic epithelial cells in the repair of brachial plexus injuries in rabbits.
RESULTS AND CONCLUSION: (1) Successfully isolated human amniotic epithelial cells expressed cytokeratin 19 rather than vimentin, and also expressed CD29 and CD73 to different extent. Scanning electron microscope observation showed human amniotic epithelial cells had filled cell bodies and were closed interconnected in good condition. (2) After 18 weeks of successful modeling, human amniotic epithelial cells labeled with green fluorescent protein were visible in the injury site under the fluorescence microscope. (3) In the experimental group, functional recovery of the rabbit forelegs was found at postoperative 6 weeks and dramatically improved at 12 and 18 weeks after surgery. Functional scores were also significantly increased in the experimental group. However, there was no improvement in the control group. These findings indicate that human amniotic epithelial cells are involved in the repair of brachial plexus injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: brachial plexus neuritis, amnion, epithelial cells, green fluorescent proteins

CLC Number:

R318

Zhang Yang, Liu Chun-xia, Wang Liang, Zhang Li, Liu Wei, Zhao Wei, Li Zhi. Human amniotic epithelial cell transplantation for repair of brachial plexus injury in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(53): 9157-9163.

Figures/Tables 4

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