Combination of bioactive glass and chitosan as a bone repair material

doi:10.3969/j.issn.2095-4344.2013.51.020

Abstract

Abstract:

BACKGROUND: Bioactive glass, a multi-phase composite material, has good biological activity, bone conductivity and biocompatibility, but as a bone repair material it cannot be completely degraded, and has low mechanical strength that is insufficient.
OBJECTIVE: To design a kind of bioactive glasses/chitosan composite scaffold, and to investigate its physicochemical properties and cell compatibility.
METHODS: Hydrochloric acid solution containing 2.0% chitosan was mixed with β-glycerophosphate at a radio of 7:1 to prepare chitosan solution. Bioactive glasses of 0.5, 1.0, 1.5 g were added into the prepared chitosan solution, and the mass ratios of chitosan and bioactive glass were 2:1, 1:1, and 1:1.5 respectively. The composite materials were immersed and mineralized in simulated body fluid for 7 days.
RESULTS AND CONCLUSION: Scanning electron microscopy showed that the composite scaffold had an interconnected porous structure with the porosity of 89% and the pore size of 100-300 μm; bioactive glasses dispersed in a needle shape between the chitosan scaffolds, arranged evenly, and were fully wrapped tightly by the scaffolds. With the increase in mass of bioactive glass, the porosity of the composites decreased, but the fracture strength gradually increased. There was a positive correlation between the composite porosity and fracture strength. X-ray diffraction and Fourier transform infrared spectroscopy confirmed that the composite scaffold appeared to have no changes in the nature of single materials, and differential scanning calorimetry analysis showed no mass loss at normal body temperature. After 3 days of mineralization, hydroxyapatite forming on the material surface gradually grew up as a villous shape, and also significantly increased in number. After 7 days of mineralization, hydroxyapatite changed from a villous shape to a needle shape, the amount of hydroxyapatite was increased further, and many mineralized products were in a spherical shape.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, tissue engineering, chitosan, stents

CLC Number:

R318

Sun Chen, Zhu Shao-bo, Yu Zhi-hong, Sun Zhi-bo, Qi Bai-wen, Zhang Tao, Jin Lin, Maihemutijiang• Muhaimaiti. Combination of bioactive glass and chitosan as a bone repair material[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(51): 8907-8913.

Figures/Tables 7

References

[1]Nakahara H,Coldberg VM,Caplan AI.Culture expanded periosteal—derived cells exigbit ostechondeogenic potential in porous calcium phosphate ceramics in vivo．Clin Orthop Relat Res.1992;(276):291-298．

[2]Borden M,Attawia M,Khan Y,et al.Tissue engineered bone formation in vivo using a novel sintered polymeric microsphere mat rix.J Bone Joint Surg Br. 2004;86(8):1200.

[3]Langer R,Vacanti JP.Tissue engineerin.Science.1993;260: 920-926.

[4]Griffith LG.Polymeric biomaterials.Acta Mater.2000;48: 263-277.

[5]Agrawal CM, Ray RB.Biodegradable polymer scaffolds for musculoskeletal tissue engineering.J Biomed Mater Res. 2001;55(2):141-150.

[6]Hayashi T.Biodegradable polymers for biomedical uses.Prog Polym Sci.1994;19: 663-702.

[7]Reis RL.Natural-based polymers for biomedical applications.Woodhead Publishing,Cambridge,2008.

[8]Lee KY,Mooney DJ.Hydrogels for tissue engineering.Chem Rev.2001;101(7):1869-1878.

[9]Varghese S,Elisseeff JH.Hydrogels for musculoskeletal tissue engineering.Adv Polym Sci.2006;203:95-144.

[10]Kokubo T, Takadama H.How useful is SBF in predicting in vivo bone bioactivity?Biomaterials.2006;27(15): 2907-2915.

[11]Bohner M, Lemaitre J.Can bioactivity be tested in vitro with SBF solution?Biomaterials.2009;30(12):2175-2179.

[12]Rohanová D, Boccaccini AR, Yunos DM,et al.TRIS buffer in simulated body fluid (SBF) distorts the assessment of glass-ceramic scaffold bioactivity.Acta Biomater.2011;7(6): 2623-2630.

[13]Huang W,Day DE,Kittiratanapiboon K,et al.Kinetics and mechanisms of the conversion of silicate (45S5), borate, and borosilicate glasses to hydroxyapatite in dilute phosphate solution.J Mater Sci Mater Med.2006;17:583-596.

[14]Huang W,Rahaman MN,Day DE,et al.Mechanisms for converting bioactive silicate, borate, and borosilicate glasses to hydroxyapatite in dilute phosphate solution.Phys Chem Glasses:Eur J Glass Sci Technol B.2006;47(6):647-658.

[15]Hench LL,Splinter RJ,Allen WC,et al.Bonding mechanisms at the interface of ceramic prosthetic materials.J Biomed Mater Res.1971;5:117-141.

[16]Hench LL,Polak JM.Third-generation biomaterials.Science. 2002; 295:1014-1017.

[17]Lai W, Garino J, Ducheyne P. Silicon excretion from bioactive glass implanted in rabbit bone.Biomaterials.2002;23:213-217.

[18]Chen ZQ,Thompson ID,Boccaccini AR.45S5 Bioglass®-derived glass-ceramic scaffold for bone tissue engineering.Biomaterials2006;27:2414-2425.

[19]Austin K.Scaffold Design: Use of Chitosan in cartilage tissue engineering.MMG 445 Basic Biotech. eJ. 2007;3(1):62.

[20]赖琛,唐绍裘.金属用耐磨耐高温陶瓷涂料的研究[J].陶瓷工程, 2000,34(6):41-43.