Chinese Journal of Tissue Engineering Research
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Hao Xiao-juan1, Zhu Lü-yun2
Revised:
2013-08-06
Online:
2013-11-05
Published:
2013-11-05
Contact:
Zhu Lü-yun, M.D., Professor, Department of Endocrinology, Bethune International Peace Hospital, Shijiazhuang 050082, Hebei Province, China
zzllyy2008@yahoo.com.cn
About author:
Hao Xiao-juan★, Master, Physician, Graduate School of Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
crazygirl1314@163.com
CLC Number:
Hao Xiao-juan, Zhu Lü-yun. Umbilical cord mesenchymal stem cells undergoing hypoxic preconditioning can differentiate into vascular endothelial-like cells[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.45.008.
2.7 诱导后的内皮样细胞形态 诱导起初各组细胞增殖程度一致,1周后单纯脐带间充质干细胞组细胞明显增殖,细胞相互融合、重叠,细胞呈长梭形;脐带间充质干细胞+氯化钴诱导3 d组细胞增殖程度稍低于单纯脐带间充质干细胞组,细胞同样呈长梭形;脐带间充质干细胞+氯化钴组与单纯脐带间充质干细胞组细胞增殖明显减低,较诱导前无明显改变,见图6。 随着时间延长,单纯脐带间充质干细胞组和脐带间充质干细胞+氯化钴诱导3 d组增殖程度逐渐减低,细胞呈多角形及长梭形;脐带间充质干细胞+氯化钴组增殖程度大于单纯脐带间充质干细胞组和脐带间充质干细胞+氯化钴诱导3 d组,细胞形态近似圆形;对照组增殖减少最明显,细胞形态无明显改变;于第5周,前3组细胞形态均呈短缩、类圆形及形态不规则形,见图7。对照组形态较前无明显变化。"
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