Number of mast cells and expression of transforming growth factor beta 1 in the submandibular gland of diabetes mellitus rats

doi:10.3969/j.issn.2095-4344.2013.24.013

Abstract

Abstract:

BACKGROUND: Continuous hyperglycemia may result in degenerative changes in submandibular gland cells, showing the increased cytokine expression, altered glycogen metabolism and attenuated secretion function. Little evidence is available addressing the mast cells and transforming growth factor beta 1 in diabetic submandibular gland lesions.
OBJECTIVE: To observe the number and morphological changes of mast cells, as well as transforming growth factor beta 1 expression in the submandibular gland of diabetic rats.
METHODS: Thirty-two male Sprague-Dawley rats were randomly divided into an experimental group and a control group. The body weight and blood glucose levels of all rats were measured at 4, 8, and 12 weeks after alloxan injection was given in the experimental group. Submandibular gland tissues were harvested for hematoxylin-eosin staining and toluidine blue staining to count mast cells. Transforming growth factor beta 1- positive cells were measured using SP immunohistochemical staining method.
RESULTS AND CONCLUSION: (1) Mast cells were expressed in submandibular gland of both normal rats and diabetic rats, and the number of mast cells gradually increased as the disease duration prolonged and was significantly higher in the experimental group than in the control group (P < 0.01). (2) The transforming growth factor beta 1-positive cells were expressed in submandibular gland acinar cells, granular convoluted tubule and striated duct cells in both normal rats and diabetic rats, and the positive granules were localized in the cytoplasm. The number of positive cells gradually increased along with the duration of diabetes and was significantly higher in the experimental group than in the control group (P < 0.01). The mast cells and upregulated expression of transforming growth factor beta 1 in the submandibular gland of diabetic rats are positively related to the duration of diabetes mellitus

Key words: tissue construction, tissue construction and bioactive factors, diabetes mellitus, transforming growth factor beta 1, mast cells, submandibular gland, rats, immunohistochemistry, toluidine blue staining, hematoxylin-eosin staining, other grants-supported paper

CLC Number:

R318

Zhu Jian-hua, Tang Chun-ling, Zhang Yan-qiu, Liu Ji-guang. Number of mast cells and expression of transforming growth factor beta 1 in the submandibular gland of diabetes mellitus rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4457-4464.

Figures/Tables 4

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