Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (38): 7121-7125.doi: 10.3969/j.issn.2095-4344.2012.38.019

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Preparation of tetracycline hydrochloride-loaded chitosan controlled-released films

Ding Man1, Wang Hua-lin1, Jiang Shao-tong2   

  1. 1School of Chemical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China
    2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China
  • Received:2011-12-31 Revised:2012-02-20 Online:2012-09-16 Published:2012-09-16
  • Contact: Wang Hua-lin, Professor, School of Chemical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China doc_hlwang@ hfut.edu.cn
  • About author:Ding Man★, Studying for master’s degree, School of Chemical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, China doc_dingman870813@163.com

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Abstract:

BACKGROUND: Chitosan has good biocompatibility, biodegradability and antibiotic activity.
OBJECTIVE: To prepare chitosan nanofiber films with various tetracycline hydrochloride contents by using tape casting method, and to investigate their performance in preventing bacterial infections as well as their controlled-release characteristic.
METHODS: Chitosan/tetracycline hydrochloride controlled-release films, 0.03 mm in thickness, with various tetracycline hydrochloride contents (0, 3%, 5%, 10%, 20%) were prepared by tape casting method. Drug loaded efficiency was determined, and drug release profiles were drawn. The in vitro antibiotic activity of drug release films was tested by using solution culture and solid culture methods. Degradation of the films was observed by phosphate buffer solution method.
RESULTS AND CONCLUSION: Drug loaded efficiencies of the chitosan/tetracycline hydrochloride films were decreased with the tetracycline hydrochloride contents increased. Besides, burst release amount was increased. The chitosan/tetracycline hydrochloride films could inhibit staphylococcus aureus growth, and with the increase of the tetracycline hydrochloride contents, their antibacterial effect was enhanced. Until the tetracycline hydrochloride content was over 10%, the antibacterial effect did not change significantly. Tetracycline hydrochloride could promote the degradation of chitosan films, and with the increased tetracycline hydrochloride contents, the rate of degradation was faster. Chitosan/tetracycline hydrochloride films with more than 10% tetracycline hydrochloride could be degraded completely within 8 days. These findings suggest that the chitosan film with over 10% tetracycline hydrochloride is a better choice considering the price and curative effect.

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