Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (35): 6560-6564.doi: 10.3969/j.issn.2095-4344.2012.35.020

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Effects of hyaluronic acid on the osteopontin and CD44 expression in fibroblast-like synoviocytes of human with knee osteoarthritis in vitro

Zhang Fang-jie, Luo Wei, Gao Shu-guang, Li Yu-sheng, Sun Jin-peng, Xiong Yi-lin, Lei Guang-hua   

  1. Department of Orthopedics, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China
  • Received:2012-01-17 Revised:2012-01-17 Online:2012-08-26 Published:2012-08-26
  • Contact: Lei Guang-hua, Professor, Doctoral supervisor, Department of Orthopedics, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China lgh9640@sina.com
  • About author:Zhang Fang-jie☆, Studying for doctorate, Department of Orthopedics, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China, zhangfangjie10@sina.com

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Abstract:

BACKGROUND: In the course of osteoarthritis, the changes of hyaluronic acid level can make a series changes of cytokines and enzyme expression levels. However, the relationship between the high expression of osteopontin and CD44 in the synovial tissue and the hyaluronic acid level change is not clear.
OBJECTIVE: To change the contents of hyaluronic acid in the fibroblast-like synoviocytes, in order to investigate the effect of hyaluronic acid on the expression of osteopontin and CD44 of fibroblast-like synoviocytes in patients with osteoarthritis of the knee.
METHODS: The study was performed on 10 specimens of synovium obtained from osteoarthritic patients. Then all the fibroblast-like synoviocytes 4-6 passaged cells were taken and divided into three groups: the control group without any treatment; hyaluronic acid group was treated with 100 mg/L hyaluronic acid for 24 hours; hyaluronidase group was treated with 300 mg/L hyaluronidase for 24 hours.
RESULTS AND CONCLUSION: Compared with control group, the osteopontin mRNA expression in osteoarthritis fibroblast-like synoviocytes was increased in hyaluronic acid group (P < 0.05) and decreased in hyaluronidase group (P < 0.05), and the difference of osteopontin mRNA expression showed statistically significance in hyaluronic acid group and hyaluronidase group (P < 0.05). The difference of the CD44 mRNA expression in fibroblast-like synoviocytes was not significant among three groups (P > 0.05). Hyaluronic acid can up-regulate the osteopontin mRNA expression in osteoarthritis fibroblast-like synoviocytes and has no effect on the CD44 mRNA expression.

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