Effects of different types of fluoride-free toothpaste on the remineralization of enamel after acid erosion: an in vivo study

doi:10.3969/j.issn.2095-4344.1727

Abstract

Abstract:

BACKGROUND: The development of the swallowing reflex in young children is incomplete, and there is a risk of fluorosis when using fluoride toothpaste. By contrast, fluoride-free toothpaste is much safer for children.

OBJECTIVE: To compare the effects of OHOLV toothpaste, GC MI paste and fluoride-free toothpaste on the remineralization of demineralized enamel in family oral care.

METHODS: Fifteen patients, aged 12-18 years, who were prepared to receive extractions of healthy first premolars (60 teeth in total) due to orthodontic needs in the Department of Stomatology of Jingmen Rehabilitation Hospital, were included. Left maxillary and mandibular first premolars as controls were extracted immediately after acid eroding on the buccal surface. Five patients were randomly selected and included into fluoride-free toothpaste group. After their right maxillary and mandibular first premolars were eroded, fluoride-free toothpaste was applied every morning and evening, and after 10 days, these teeth were extracted. Five patients were randomly selected and included into OHOLV toothpaste group. After their right maxillary and mandibular first premolars were eroded, OHOLV toothpaste was applied every morning and evening, and after 10 days, these teeth were extracted. Similarly, for the five patients in the GC MI paste group, the same erosion was performed before the application of GC MI paste for 3 minutes every morning and evening, and after 10 days, these teeth were also extracted. Buccal enamel specimens were isolated, observed and analyzed by scanning electron microscopy and energy-dispersive X-ray spectroscopy.

RESULTS AND CONCLUSION: (1) Scanning electron microscope showed that the enamel surface in the control group was irregular, porous and honeycomb-shaped, the center of the enamel rod dissolved, and many porous defects appeared. A few scattered mineral deposits could be seen on the enamel surface in the fluoride-free toothpaste group. There were still a lot of honeycomb-shaped pores on the enamel surface in the OHOLV toothpaste and GC MI paste groups. Meanwhile, there were also a large number of mineral deposits and the pores became smaller. The mineralized particles were small and the deposits were uneven. The GC MI paste group had a larger amount of deposits. (2) Energy-dispersive X-ray spectroscope showed no difference in calcium and phosphorus levels between control and fluoride-free toothpaste groups (P > 0.05). No difference was observed in the calcium and phosphorus levels between OHOLV toothpaste and GC MI paste groups (P > 0.05). The OHOLV toothpaste and the GC MI paste groups had higher calcium and phosphorus levels than the control and fluoride-free toothpaste groups (P < 0.05). (3) These results suggest that OHOLV toothpaste and GC MI paste can significantly promote the remineralization of demineralized enamel, especially GC.

Key words: toothpaste, bioactive glass, casein phosphopeptides, amorphous calcium phosphate, OHOLV toothpaste, fluoride-free toothpaste, remineralization

CLC Number:

R459.9

Wu Tingyun, Wang Detang, Zhu Youjia, Ruan Qiong, Wu Aimin, He Shangqun, Zeng Xiaofang. Effects of different types of fluoride-free toothpaste on the remineralization of enamel after acid erosion: an in vivo study[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(18): 2842-2846.

Figures/Tables 2

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