Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (18): 2871-2879.doi: 10.3969/j.issn.2095-4344.1653

Previous Articles     Next Articles

Physicochemical properties and cellularization of hydroxyapatite/zirconium dioxide foam ceramics

Chai Le1, Quan Renfu2, Lü Jianlan1, Huang Xiaolong2, Zhang Can1, Ren Weifan1, Qian Jiansheng1
  

  1. 1Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China; 2Jiangnan Hospital, Zhejiang Chinese Medical University, Hangzhou 311200, Zhejiang Province, China
  • Received:2018-11-08 Online:2019-06-28 Published:2019-06-28
  • Contact: Quan Renfu, Chief physician, Jiangnan Hospital, Zhejiang Chinese Medical University, Hangzhou 311200, Zhejiang Province, China
  • About author:Chai Le, Master candidate, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China
  • Supported by:

    the Medical and Health Scientific Program of Zhejiang Province, No. 2014KYA191 (to QRF); the Major Scientific Research Fund of Zhejiang Province, No. 2014C03031 (to QRF)

Abstract:

BACKGROUND: Studies have confirmed that hydroxyapatite/zirconium dioxide foam ceramic materials have the ability to promote bone regeneration, but their genotoxicity has not been reported.
OBJECTIVE: To detect the porosity, compressive strength, genotoxicity and cytotoxicity of hydroxyapatite/zirconium dioxide foam ceramics and to explore the osteogenic differentiation of artificially induced pluripotent stem cells cultured on the hydroxyapatite/zirconium dioxide foam ceramics.
METHODS: The hydroxyapatite/zirconium dioxide foam ceramics was prepared by high temperature sintering method with addition of pore-forming agent. The porosity and compressive strength of hydroxyapatite/zirconium dioxide foam ceramics were detected, and the genotoxicity of the material were tested using Salmonella typhimurium reverse mutation test. L929 cells were cultured with hydroxyapatite/zirconium dioxide foam ceramics extract (experimental group), phenol solution (positive control group), and normal saline (negative control group). After 24 hours of culture, the cytotoxicity was detected using cell counting kit-8 toxicity test. Artificially induced pluripotent stem cells-derived mesenchymal stem cells were inoculated onto the hydroxyapatite/zirconium dioxide foam ceramics. On the 3rd, 7th, 10th and 14th days of culture, the amount of alkaline phosphatase secreted by the cells was detected. On the 14th day of culture, the expression of type I collagen in the cells was detected by immunohistochemistry, and cell adhesion was observed by scanning electron microscope.
RESULTS AND CONCLUSION: (1) The porosity and compressive strength of hydroxyapatite/zirconium dioxide foam ceramics were (76.72±0.75)% and (11.60±1.35) MPa, respectively. (2) In the Salmonella typhimurium reverse mutation test, the metabolites of hydroxyapatite/zirconium dioxide foam ceramics were not mutagenic or carcinogenic. (3) In the cell counting kit8 test, the survival rate of the cells in the experimental group was close to that in the negative control group (P > 0.05), but still significantly higher than that in the positive control group (P < 0.05). (4) With the extension of culture time, the secretion amount of alkaline phosphatase was increased gradually, and there was significant difference at different observational time points (P < 0.05). (5) On the 14th day of culture, a great amount of artificially induced pluripotent stem cells-derived mesenchymal stem cells evenly distributed and grew well on the surface of hydroxyapatite/zirconium dioxide foam ceramics, and these cells had good morphology and highly expressed type I collagen. To conclude, the hydroxyapatite/zirconium dioxide foam ceramics has a certain compressive capacity and does not exhibit mutagenic effects and cytotoxicity, and promote the osteogenic differentiation of artificially induced pluripotent stem cells-derived mesenchymal stem cells cultured on its surface.

Key words: hydroxyapatite, zirconium dioxide, compressive strength, genotoxicity, cytotoxicity, osteogenic differentiation, cytotoxicity test, type I collagen expression in complex cells

CLC Number: