Abstract:

BACKGROUND: As for traditional cryopreserving method, cells are cooling by steps at 4 ℃ and -20 ℃, which is complicated and a waste of time.
OBJECTIVE: To compare the protective effects of traditional and reforming cryopreserving methods on human vascular endothelial cells.
METHODS: The vascular endothelial cells were incubated with a freezing medium consisting of 10% dimethyl sulfoxide, 60% fetal bovine serum and 30% DMEM serum-free medium after digested with trypsin, and then the cell suspension were put into preserving tubes. Each group was intervened. In the experimental group, the tubes were packaged with gauze and then thawed into -80 ℃ refrigerator straightly. In the control group, the tubes were pre-cooling at 4 ℃ and -20 ℃ for 30 minutes and 1 hour, then thaw into -80 ℃ refrigerator. One month later, the endothelial cells were resuscitated.
RESULTS AND CONCLUSION: There were no significant difference in the survival rates and growth curves between experimental group and control group. The experimental group was significantly better than control group in adherence rates, morphological changes and proliferation. The reforming cryopreserving method is better than traditional cryopreserving method which is more convenient and easier to operate.