Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9579-9582.doi: 10.3969/j.issn.1673-8225.2011.51.019

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Preparation and characterization of biocompatible Fe3O4@SiO2 nanoparticals

Sun Peng-fei1, Xie Ke-rong1, Zhang Feng-wei2, Jiao Zong-xian3, Cao Xiang-rong1   

  1. 1Lanzhou University Second Hospital, Lanzhou  730030, Gansu Province, China; 2College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou  730000, Gansu Province, China; 3College of Basic Medical Science, Lanzhou University, Lanzhou  730000, Gansu Province, China
  • Received:2011-05-17 Revised:2011-07-27 Online:2011-12-17 Published:2011-12-17
  • About author:Sun Peng-fei☆, Doctor, Associate chief physician, Associate professor, Master’s supervisor, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China sunpengfeiyy@ msn.com
  • Supported by:

    the Fundamental Research Funds for the Central Universities in Lanzhou University, No. lzujbky-2009-154*

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Abstract:

BACKGROUND: Fe3O4 nanoparticles have good magnetic properties, SiO2 has good biocompatibility, and Fe3O4@SiO2 composite nanoparticles are expected to become the carrier of targeted therapy.
OBJECTIVE: To prepare the biocompatible Fe3O4@SiO2 nanoparticles via a reverse microemulsion.
METHODS: Firstly, hydrophobicity Fe3O4 nanoparticle modified oleic acid were prepared via a one-pot chemical coprecipitation with FeCl3•6H2O, FeCl2•4H2O, oleic acid and NH3•H2O. Subsequently, Fe3O4 nanoparticles stabilized with oleic acid were dispersed in cyclohexane, and then Triton-X100, hexylalcohol and H2O were added into the above solution and stirred to form stable reverse microemulsion; In the reverse microemulsion, using ammonia solution as a catalyst, tetraethyl orthosilicate (TEOS) was hydrolyzed and condensed to form Fe3O4@SiO2 compound nanoparticles.
RESULTS AND CONCLUSION: ①The structure of Fe3O4 nanopartides prepared by a one-pot chemical coprecipitation was spinel, having a mean diameter of 3.5 nm; Through microemulsion methods, SiO2 were coated successfully on the surface of Fe3O4 nanopartides, and the uniform Fe3O4@SiO2 nanoparticles with a mean diameter of 40 nm were obtained. ②Although the saturation magnetization decreased after Fe3O4 nanopartides being coated, the coercivity of magnetic nanoparticles was near zero before and after encapsulation, revealing Fe3O4 and Fe3O4@SiO2 nanoparticles are of superparamagnetic properties.③After EA. hy926 cells were cultured accompanied with Fe3O4@SiO2 nanoparticals, the absorbance value of Fe3O4@SiO2 nanoparticles was markedly higher than that of control group (P < 0.05); At 48 and 72 hours of cell culture, no significant difference was found in the absorbance value between the two group (P > 0.05). The results showed Fe3O4@SiO2 compound nanoparticle using a reverse microemulsion is a kind of excellent biomaterial because of its stable, dispersive and superparamagnetic properties.

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