Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9535-9538.doi: 10.3969/j.issn.1673-8225.2011.51.009

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Feasibility of biodegradable polyurethane in construction of tissue engineering anterior cruciate ligament

Liu Long-fei1, 2, Zhang Wen-tao1, Huang Wei1, Zhang Xin-tao1, Xiao De-ming1   

  1. 1Peking University Shenzhen Hospital, Shenzhen  518036, Guangdong Province, China
    2Anhui Medical University, Hefei  230032, Anhui Province, China
  • Received:2011-09-07 Revised:2011-10-13 Online:2011-12-17 Published:2011-12-17
  • Contact: Zhang Wen-tao, Doctor, Master’s supervisor, Chief physician, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong Province, China zhangwt2007@ gmail.com
  • About author:Liu Long-fei★, Studying for master’s degree, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong Province, China; Anhui Medical University, Hefei 230032, Anhui Province, China llf8421@163.com
  • Supported by:

    Guangdong Natural Science Foundation, No. 8151803601000013*

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Abstract:

BACKGROUND: The anterior cruciate ligament (ACL) of the knee joint is the common site of injury. Tissue engineering has achieved much progress in the past 10 years for ACL, but none of the ideal scaffolds.
OBJECTIVE: To investigate the feasibility of biodegradable polyurethane in construction of tissue engineering ACL.
METHODS: Leaching liquor of polyurethane composed of biodegradable polyurethane fibers was prepared on DMEM medium containing fetal bovine serum. NIH3T3 cells were cultured in DMEM medium with 100%, 50%, 25%, and 12.5% of leaching liquor. The cytotoxicity of polyurethane was detected by CCK-8 agent. Rabbit ACL cells and NIH3T3 cells were isolated and cultured in vitro, and seeded onto the surface of the polyurethane scaffold to observe their growth. The biomechanical properties of the polyurethane scaffold were measured and analyzed with electronic tensile machine.
RESULTS AND CONCLUSION: The braided polyurethane scaffold materials had no cytotoxicity; ACL and NIH3T3 cells adhered, grew and proliferated well on the surface of the braided polyurethane scaffold materials. The Young’s modulus, ultimate tensile stress and elongation at break were (41.2±2.1) MPa, (51.0±1.5) MPa and (600±60)% respectively. The polyurethane scaffold materials possess good biomechanical properties and biological safety.

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