Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9373-9376.doi: 10.3969/j.issn.1673-8225.2011.50.016

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Effects of intrathecal Sufentanil injection on the expression of N-methyl-D-aspartate receptor and calcitonin gene-related peptide in the spinal dorsal horn of rat bone cancer pain models 

Wang Yi-chun1, Wang Ming-de2, Li Zu-rong1, Liu Jing-shi2, Yang Wen-qian2   

  1. 1Department of Critical Care Medicine, 2Department of Anesthesiology, Hunan Provincial Tumor Hospital, Changsha  410013, Hunan Province, China
  • Received:2011-08-31 Revised:2011-10-25 Online:2011-12-10 Published:2011-12-10
  • About author:Wang Yi-chun☆, Studying for doctorate degree, Associate chief physician, Department of Critical Care Medicine, Hunan Provincial Tumor Hospital, Changsha 410013, Hunan Province, China wangyichun2005@sina.com
  • Supported by:

    the Science and Technology Bureau of Hunan Province, No. 2009SK3112*; the Health Bureau of Hunan Province, No. C2008-005*

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Abstract:

BACKGROUND: There are considerable animal experiments and clinical evidence indicating obvious analgesic effects of intrathecal Sufentanil injection, but the mechanism behind this is unclear.
OBJECTIVE: To investigate the effects of intrathecal Sufentanil injection on the expression of N-methyl-D-aspartate recaptors and calcitonin gene-related peptide in spinal dorsal horn of rat bone cancer pain models.
METHODS: A total of 36 healthy male Sprague-Dawley rats were randomly divided into four groups: control group, sham operation group, bone cancer pain model group and Sufentanil group. Bone cancer pain model in the latter two groups was constructed using ascitic fluid of breast cancer cells. Rats in sham operation group were injected with heat inactivated dead cells. Rats in model group and Sufentanil group were treated with saline and Sufentanil daily by intrathecal injection within 15 days after model construction.
RESULTS AND CONCLUSION: Compared with control group and sham operation group, the expression of N-methyl-D-aspartate recaptors and calcitonin gene-related peptide in spinal dorsal horn increased in model group on the 12th and 14th days after model construction (P < 0.01). The mechanical allodynia pain threshold and radiant heat stimulus paw withdrawal latency decreased in model group (P < 0.01). Compared with model group, the expression of N-methyl-D-aspartate recaptors and calcitonin gene-related peptide in the superficial layer of spinal dorsal horn decreased on the 12th and 14th days after injection (P < 0.01). The mechanical allodynia pain threshold and radiant heat stimulus paw withdrawal latency increased (P < 0.01). It suggests that intrathecal administration of Sufentanil provide a significant antinociception effect in rat bone cancer pain models, and the mechanisms behind this is related to the inhibition of N-methyl-D-aspartate recaptors and calcitonin gene-related peptide in rat spinal dorsal horn.

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