Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9353-9355.doi: 10.3969/j.issn.1673-8225.2011.50.011

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Effect of continuous static hydrostatic pressure on cell proliferation

Guo Zi-fen, Feng Yong, Li Kai, Liao Duan-fang   

  1. Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China
  • Received:2011-06-22 Revised:2011-07-01 Online:2011-12-10 Published:2011-12-10
  • Contact: Liao Duan-fang, Doctor, Professor, Doctoral supervisor, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China dfliao66@yahoo.com.cn
  • About author:Guo Zi-Fen☆, Doctor, Associate professor, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, Hunan Province, China guozifen@yahoocom.cn
  • Supported by:

    the National High Technology Research and Development Program of China (863 Program), No. 2008AA02Z436*; the National Natural Science Foundation of China, No. 81102516*; the Graduate Innovation Project of Hunan Province, No. CX2009B191*

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Abstract:

BACKGROUND: Vascular smooth muscle cells grow well in culture condition using static hydrostatic pressure instead of serum.
OBJECTIVE: To investigate the effect of continuous static hydrostatic pressure on the proliferation of vascular smooth muscle cells.
METHODS: Vascular smooth muscle cell A10 and vascular endothelial cell HUVEC-12 were cultured in a self-developed pressure adjustable incubator, under different pressures of 0, 12, 16, 20, 24 and 28 kPa respectively.
RESULTS AND CONCLUSION: The most obvious proliferation of A10 cell was detected under the pressure of 16 kPa, with greatest cell activity. And the most obvious proliferation of HUVEC-12 cell was under 20 kPa pressure, with greatest cell activity. It confirms that limited hydrostatic pressure can promote the proliferation of A10 and HUVEC-12 and increase the cell activity.

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