Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9344-9347.doi: 10.3969/j.issn.1673-8225.2011.50.009

Previous Articles     Next Articles

Interaction between primary cultured human thymocytes and human immunodeficiency virus

Gao Nan-jun1, Chen Ying-jie2, Li Xue-tao3, Kong Wei-yun3, Zhou Yi-ping4   

  1. 1Department of Pediatrics, Yuxi Third People's Hospital, Yuxi  653100, Yunnan Province, China
    2Department of Human Anatomy, Kunming Medical University, Kunming  650031, Yunnan Province, China
    3Department of Orthopedics, Second People’s Hospital of Yunnan Province, Kunming  650051, Yunnan Province, China
    4Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming  650031, Yunnan Province, China
  • Received:2011-09-06 Revised:2011-10-09 Online:2011-12-10 Published:2011-12-10
  • Contact: Zhou Yi-ping, Doctor, Associate professor, Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650031, Yunnan Province, China 1401545363@qq.com Correspondence to: Kong Wei-yun, Studying for doctorate, Attending physician, Department of Orthopedics, Second People’s Hospital of Yunnan Province, Kunming 650051, Yunnan Province, China kongweiyunkm128@126.com
  • About author:Gao Nan-jun, Associate chief physician, Department of Pediatrics, Yuxi Third People's Hospital, Yuxi 653100, Yunnan Province, China 761470408@qq.com Chen Ying-jie, Master, Professor, Department of Human Anatomy, Kunming Medical University, Kunming 650031, Yunnan Province, China chenyj2002@foxmail.com Gao Nan-jun and Chen Ying-jie contributed equally to this paper.
  • Supported by:

    the Foundation of Yunnan Provincial Science and Technology Committee, No. 98C014Q*; the Joint Specific Foundation of Yunnan Provincial Science and Technology Committee and Kunming Medical University, No. 2007C0044R*, 2009CD188*; the Innovation Foundation of Yunnan Provincial Universities for Key Pharmaceutical Laboratory, No. 6010902009-2010ZL004*; the Scientific Research Program of Education Bureau of Yunnan Province, No. 2010Y191*, 07Z10199*

PDF

393

Abstract:

BACKGROUND: Human immunodeficiency virus binds to CD4 receptors in human thymocytes, and thus causes activity decreasing in CD4 cells.
OBJECTIVE: To explore the relationship between human embryo thymocytes and human immunodeficiency virus.
METHODS: Primary cultured human embryo thymocytes were divided into experiment group and control group. Experimental thymocytes were mixed cultured with human immunodeficiency virus 1 Ⅲ B. Control thymocytes were cultured without human immunodeficiency virus.
RESULTS AND CONCLUSION: According to transmission electron microscopy, massive human immunodeficiency virus particles emerged in thymocytes treated with human immunodeficiency virus at the 40th hour after cultivation. According to MTT assay and immunohistochemical staining, the absorbance in thymocytes treated with human immunodeficiency virus was significantly lower than that in the control thymocytes at the 40th hour to the 2nd day after cultivation (r=0.9733, P < 0.05). The Fas-L positive rate of the experiment thymocytes treated with human immunodeficiency virus increased on cell membranes and in cytoplasm (P < 0.05). These findings indicate that human immunodeficiency virus decreases the cell activity in primary cultured human embryo thymocytes.

CLC Number: