Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (49): 9190-9193.doi: 10.3969/j.issn.1673-8225.2011.49.016

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Bone marrow mesenchymal stem cells improve cardiomyocyte contractility in rats with heart failure

Wang Tong, Zheng Shao-xin, Zhou Chang-qing, Weng Yin-lun, Wen Zhu-zhi   

  1. Emergency Department, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou  510120, Guangdong Province, China
  • Received:2011-06-19 Revised:2011-11-01 Online:2011-12-03 Published:2011-12-03
  • About author:Wang Tong☆, Doctor, Associate chief physician, Associate professor, Emergency Department, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China tongwang163@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 810701258*; Science and Technology Foundation in Guangdong Province, No. 2010B031600032*; Natural Science Foundation of Guangdong Province, No. 8151008901000119*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) transplantation can significantly improve cardiac function.
OBJECTIVE: To investigate the effect of BMSCs on cardiomyocytes contractility and dilatation in rats with heart failure after myocardial infarction.
METHODS: Left anterior descending coronary arteries of 10 Sprague Dawley rats were ligated in order to establish the myocardial ischemia model. The model rats were randomly divided into two groups: the experimental group and the control group. Four weeks later, 5×106/L PKH26 labeled BMSCs were injected into the infracted myocardium in the experimental group and in the control group, only injected with PBS.  
RESULTS AND CONCLUSION: Compared with ligation, the cardiac function in two groups after modeling for 4 weeks was improved significantly (P < 0.01). After treated with BMSCs administration, the cardiac function in experimental group was improved significantly (P < 0.05). The amplitude of shortening/relengthening and contractile velocity of cardiomyocytes in experimental group was significantly increased (P < 0.01). The cardiomyocytes with contractility differentiated from BMSCs could be seen under fluorescence microscope. The injection of BMSCs could significantly increase the shortening/relengthening function of rat cardiomyocytes and was one of the important mechanisms in BMSCs improved of heart failure rats.

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