Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8643-8647.doi: 10.3969/j.issn.1673-8225.2011.46.022

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Repeated rapid skin expansion promotes the expression of epidermal growth factor and proliferating cell nuclear antigen

Ren Gui-yun1, Wang Shou-jun2, Dong Fu-sheng1, Yu Mei-qing1, Hao Fu-liang1, Zhang Xu-dong1   

  1. 1Department of Oral and Maxillafical Surgery, School of Stomatology, Hebei Medical University, Hebei Province Key Laboratory of Stomatology, Shijiazhuang  050017, Hebei Province, China
    2Funing County People’s Hospital of Hebei Province, Qinhuangdao   066300, Hebei Province, China
  • Received:2011-05-09 Revised:2011-06-16 Online:2011-11-12 Published:2011-11-12
  • Contact: Dong Fu-sheng, Professor, Department of Oral and Maxillafical Surgery, School of Stomatology, Hebei Medical University, Hebei Province Key Laboratory of Stomatology, Shijiazhuang 050017, Hebei Province, China dongfsh@126.com
  • About author:Ren Gui-yun☆, Studying for doctorate, Associate professor, Department of Oral and Maxillafical Surgery, School of Stomatology, Hebei Medical University, Hebei Province Key Laboratory of Stomatology, Shijiazhuang 050017, Hebei Province, China
  • Supported by:

     Science and Technology Support Plan Program of Hebei Province, No. 08276101D-59*

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Abstract:

BACKGROUND: Repeated rapid skin expansion has been proposed because of deficiency in conventional expansion and rapid expansion currently used in the clinic.
OBJECTIVE: To investigate the effect of the repeated rapid skin expansion on the expression of epidermal growth factor (EGF) and proliferating cell nuclear antigen (PCNA).
METHODS: Forty-eight male rabbits were randomly divided into three groups: repeated rapid expansion, rapid expansion, conventional expansion (16 rabbits per group). The samples were collected at different phases and were fixed in 10% formaldehyde solution. The expression of EGF and PCNA was observed with immunohistochemistry.
RESULTS AND CONCLUSION: At the 2nd and 3rd weeks, EGF expression was significantly greater in the repeated rapid expansion group than in the rapid expansion group and conventional expansion group (P < 0.05). PCNA positive staining was significantly higher in the repeated rapid expansion group than in the other two groups at each time stage except for the 3rd week (P < 0.05). These findings suggest that repeated rapid skin expansion can promote the proliferation of fibroblasts and epidermal cells by raising the content of EGF in the expanded skin.

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