Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (42): 7831-7834.doi: 10.3969/j.issn.1673-8225.2011.42.010

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Cellular compatibility of a nano-hydroxyapatite/collagen scaffold

Liu Peng, Wang Dong, Sun Hai-yu, Liu Liang, Li Shu-wei, He Jin-dong   

  1. Second Hospital Affiliated to Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2011-04-06 Revised:2011-06-22 Online:2011-10-15 Published:2011-10-15
  • Contact: Wang Dong, Professor, Doctoral supervisor, Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi Province, China Wangd080@sina.com
  • About author:Liu Peng★, Studying for master’s degree, Physician, Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi Province, China doubleidiot@163.com

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Abstract:

BACKGROUND: Under the theory of bone-tissue engineering, the biocompatibility of biomaterial scaffolds is evaluated by observing the morphology, proliferation and differentiation of osteoblasts on the biomaterials.
OBJECTIVE: To observe the effects of nano-hydroxyapatite/collagen (nHAC) scaffold on the proliferation and differentiation of osteoblasts.
METHODS: The rat osteoblasts were obtained from the cranium of newborn Wistar rats within 24 hours, and primarily cultured using modified collagenase digestion. The cells at passage 3 were co-cultured with nHAC in vitro. At 3, 6 and 9 days of the culture, cell morphology was observed by inverted phase-contrast microscopy. In addition, MTT assay and alkaline phosphatase (ALP) activity test were used to observe the effects of the material on cell differentiation and proliferation. 
RESULTS AND CONCLUSION: Osteoblasts can adhere, grow, differentiate and proliferate better on nHAC than HA. The composite scaffold nHAC has a good compatibility, indicating that the material has a great potential for application in bone tissue engineering.

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