Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5797-5800.doi: 10.3969/j.issn.1673-8225.2011.31.023

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Effects of a new immunodepressant J2 on lymphocytic secretion of interleukin 10 and interferon gamma in mice after corneal allograft

Wang Da-jiang1, Guo Hui-ling1, Huang Yi-fei1, Chen Guo-jiang2, Zhang Han3, Li Yan2   

  1. 1Department of Ophthalmology, General Hospital of Chinese PLA, Ophthalmic Center of Chinese PLA, Beijing  100853, China
    2Department of Molecular Immunology, Academy of Military Medical Sciences, Beijing  100854, China
    3Ophthalmology Center, Shandong University, Jinan  250033, Shandong Province, China
  • Received:2011-01-13 Revised:2011-03-24 Online:2011-07-30 Published:2011-07-30
  • Contact: Huang Yi-fei, Doctor, Doctoral supervisor, Chief physician, Professor, Department of Ophthalmology, General Hospital of Chinese PLA, Ophthalmic Center of Chinese PLA, Beijing 100853, China 3301yk@sina.com
  • About author:Ophthalmology, General Hospital of Chinese PLA, Ophthalmic Center of Chinese PLA, Beijing 100853, China wangdajiangv@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30973245; the General Program of China Postdoctoral Science Foundation, No. 20090451511

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Abstract:

BACKGROUND: J2 is a new immunodepressant targeting CD4, and previous studies have verified the effect of J2 on spleen cells of normal mice or mice undergoing corneal transplantation.
OBJECTIVE: To investigate the effects of J2 on the lymphocytic secretion of interferon gamma (IFN-γ) and interleukin 10 (IL-10) of mice with allogeneic corneal transplantation.
METHODS: Corneal transplantation models were created in the right eyes of 36 clean BalB/c(H2d) mice (receiptors) and 17 clean C57BL/6(H2b) mice (donors). The models were randomly divided into four groups according to the different intervene methods. Corneal opacification and neovascularization were scored. The proliferation of spleen cells of mice was detected by ATPase method, and secretion of IL-10 and IFN- γ in cytosupernatant before and after ConA stimulated were assessed by ELISA. 
RESULTS AND CONCLUSION: J2 arrest the occurrence of corneal rejection by inhibiting the activation of CD4+ T cells and suppress the proliferation and ConA-stimulated Th1 cytokine production of spleen cells.

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