Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5765-5768.doi: 10.3969/j.issn.1673-8225.2011.31.015

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Application of urine IP-10 and Fractalkine in early diagnosis of acute rejection following renal transplantation

Wang Min, Tian Pu-xun, Ding Chen-guang, Jin Zhan-kui, Ge Guan-qun, Jia Li-ning, Ruan Dong-li, Hao Jun-jun   

  1. Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an  710061, Shaanxi Province, China
  • Received:2011-01-10 Revised:2011-04-20 Online:2011-07-30 Published:2011-07-30
  • Contact: Tian Pu-xun, Doctor, Chief physician, Doctoral supervisor, Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China yuantian@mail.xjtu. edu.cn
  • About author:Wang Min★, Studying for master’s degree, Department of Renal Transplantation, Center of Nephropathy, the First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China minwang229@126. com
  • Supported by:

    the National Key Technology R&D Program of China during the Eleventh Five-year Plan, No. 2008BAI60B04*

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Abstract:

BACKGROUND: Studies have confirmed that urine IP-10 and Fractalkine play an important role in acute rejection following organ transplantation.
OBJECTIVE: To investigate early diagnosis and post-treatment expression of urine IP-10 and Fractalkine in the acute rejection after renal transplantation, through detecting the association of the urine IP-10 and Fractalkine variation according to some cases of nephridial tissue biopsy.
METHODS: According to the postoperative clinical manifestation, laboratory examination and renal biopsy histopathological examination results, we divided 106 patients with renal transplantation allograft patients into acute rejection group (n  = 16) and the stable group (n  = 90). Healthy volunteers were chosen as normal control group (n = 10). IP-10 and Fractalkine concentration changes were measured by double antibody sandwich enzyme-linked immunosorbent adsorption.
RESULTS AND CONCLUSION: The urine level of IP-10 and Fractalkine in patients with renal transplantation post-operation was significantly higher than pre-operation group. But one week post-operative, the urine level of IP-10 and Fractalkine was declined in the stable group and to preoperative level after 11 days, and acute rejection group continued high expression. IP-10 with first day postoperative and Fractalkine with third day postoperative compared with stable group showed significant difference  (P < 0.05). It is confirmed that to detect the urine levels of IP-10 and Fractalkine in patients with renal transplantation pre-operation and post-operation has certain clinical significance to early diagnose and treat acute rejection.

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