Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5731-5734.doi: 10.3969/j.issn.1673-8225.2011.31.007

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Effect of laser drilling before vitrified cryopreservation on transplantation of frozen-thawed blastocyts

Fang Jian-ye, Yang Jing, Yin Tai-lang, Cheng Yan, Zhao Qing-hong, Zou Yu-jie, Huang Wei   

  1. Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
  • Received:2011-03-29 Revised:2011-05-18 Online:2011-07-30 Published:2011-07-30
  • Contact: Yang Jing, Doctor, Professor, Doctoral supervisor, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China dryangqing@hotmail.com
  • About author:Fang Jian-ye★, Studying for master’s degree, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

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Abstract:

BACKGROUND: A series of studies have demonstrated that artificially reduced blastocoelic cavity can greatly increase the vitrified frozen-thawed effects of blastocytes, but different physical methods and materials used during the process of reduction can lead to mechanical injury to blastocytes.
OBJECTIVE: To investigate the effects of laser drilling before vitrified cryopreservation of transplantation of frozen-thawed blastocyts.
METHODS: Blastocytes from three dysgenesia patients who averaged 30 years old were subjected to laser drilling before vitrified cryopreservation to reduce the blastocoelic cavity for 1 year on average. Before transplantation, these blastocytes were thawed.
RESULTS AND CONCLUSION: Two patients acquired satisfactory pregnancy and one patient did not get pregnancy. The results showed that laser drilling before vitrified cryopreservation reduces blastocoelic cavity and transplantation of frozen-thawed blastocytes is safe and effective.

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