Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (31): 5701-5706.doi: 10.3969/j.issn.1673-8225.2011.31.001

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Correlation of tumor necrosis factor-alpha and interleukin-10 expression to acute rejection in complex tissue and serum of rats after laryngeal transplantation

Yuan Mu1, Chen Gang2, Zhao Hua2, Jing Jian-jun2   

  1. 1Outpatient Department, Xinjiang Military Area Command of Chinese PLA, Urumqi  830002, Xinjiang Uygur Autonomous Region, China
    2Department of Otorhinolaryngology, Urumqi General Hospital of Lanzhou Military Area Command of Chinese PLA, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-03-04 Revised:2011-06-19 Online:2011-07-30 Published:2011-07-30
  • Contact: Chen Gang, Doctor, Attending physician, Department of Otorhinolaryngology, Urumqi General Hospital of Lanzhou Military Area Command of Chinese PLA, Urumqi 830000, Xinjiang Uygur Autonomous Region, China chengangxf@sina. com
  • About author:Yuan Mu, Attending physician, Outpatient Department, Xinjiang Military Area Command of Chinese PLA, Urumqi 830002, Xinjiang Uygur Autonomous Region, China 13039493151@163.com

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Abstract:

BACKGROUND: It remains unclear whether proinflammatory factor, such as tumor necrosis factor-α (TNF-α), γ-interferon and anti-inflammatory cytokine, such as interleukin-10 (IL-10), interleukin-4 (IL-4) levels can change after laryngeal transplantation.
OBJECTIVE: To explore the expression of TNF-α and IL-10 in different expressive tissue layers and its relationship during the acute rejection episodes, and to evaluate the role of TNF-α and IL-10 levels in serum for prediction of early acute rejection after laryngeal transplantation.
METHODS: Laryngeal heterotopic transplantations were performed in Wistar and Sprague-Dawley rats. According to different dosages of immunodepressant, all recipients were divided into three groups: Group 0 mg, Group 5 mg, and Group 10 mg. Untreated Sprague-Dawley rats served as normal control group.
RESULTS AND CONCLUSION: Changes in serum TNF-α and IL-10 levels at postoperation days 3, 7 and 11 were positively correlated with these expressions in the epithelium mucosa and submucosa at various time points after transplantation. These indicate that the high-antigenicity of graft mainly concentrates on the layers of mucosa and submucosa. TNF-α and IL-10 concentrations can serve as indexes for predicting acute rejection after laryngeal transplantation.

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