Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3851-3854.doi: 10.3969/j.issn.1673-8225.2011.21.012

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Cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on osteoblasts in vitro

Wang Zhong-lei, Gao Yan, Lai Chun-hua, Lu Hai-bin, Li Shao-bing, Jiang Ying, Wei Cong-yun, Zhou Lei   

  1. The Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou  510280, Guangdong Province, China
  • Received:2011-02-24 Revised:2011-04-25 Online:2011-05-21 Published:2011-05-21
  • Contact: Zhou Lei, Doctor, Chief physician, Doctoral supervisor, the Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China zho668@263.net
  • About author:Wang Zhong-lei★, Studying for master’s degree, the Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China wangzhl_2008@sina.com
  • Supported by:

    the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period, No. 2007BA I18BO6*

Abstract:

BACKGROUND: Amphiphilic block copolymers have been widely applied in the drug delivery system because of the high capacity of drug carriers, nanoparticles and long circulation time.
OBJECTIVE: To evaluate the cytotoxicity effect of MePEG-PLA nanoparticles prepared by modified self-emulsion solvent diffusion technique on MG63 in vitro.
METHODS: MePEG-PLA nanoparticles were prepared by modified self-emulsion solvent diffusion technique. The cytotoxicity effect of MePEG-PLA nanoparticles on MG63 was detected using MTS assay at days 1, 2, 3. Particles size, polydispersity index and Zeta electric potential were measured by laser particle size analzer. The morphology of MePEG-PLA nanoparticles were observed by transmission electron microscope. Absorbance values at days 1, 2, 3 were detected by microplate reader.
RESULTS AND CONCLUSION: MePEG-PLA nanoparticles had a regular spherical shape with the mean diameter of 25.7 nm and scattered evenly. The Zeta potential was -8.06 mV. The cytotoxicity grade of MePEG-PLA nanoparticles was 0. The results indicate that MePEG-PLA nanoparticles prepared easily by modified self-emulsion solvent diffusion technique are nontoxic to the MG63 and have a good prospect.

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