Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3815-3821.doi: 10.3969/j.issn.1673-8225.2011.21.005

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Effect of calcium acid phosphate and calcium sulfate on tendon-bone healing

Ni Feng, Huangfu Xiao-qiao, Zhao Jin-zhong, Sha Lin, Xie Guo-ming   

  1. Department of Arthroscopic Surgery, Affiliated Sixth People's Hospital, Shanghai JiaoTong University, Shanghai  200233, China
  • Received:2010-11-28 Revised:2011-02-09 Online:2011-05-21 Published:2011-05-21
  • Contact: Huangfu Xiao-qiao, Master, Associate chief physician, Department of Arthroscopic Surgery, Affiliated Sixth People's Hospital, Shanghai JiaoTong University, Shanghai 200233, China hopejoy@163.com
  • About author:Ni Feng★, Studying for master’s degree, Physician, Department of Arthroscopic Surgery, Affiliated Sixth People's Hospital, Shanghai JiaoTong University, Shanghai 200233, China drmatrix@sina.com

Abstract:

BACKGROUND: The reports are related to the promotion of tendon-bone healing are many, the main method is to give tendon-bone gap some excitor substance, so as to promote tendon-bone healing. Calcium acid phosphate as bioactive material has bone conductibility, and has been commonly used in substitution and padding of clinical bone defect. However, calcium sulfate as artificial material has potential osteoinductivity.
OBJECTIVE: To observe the effect of calcium acid phosphate and calcium sulfate on tendon-bone healing during the process of anterior cruciate ligament of knee joint constructed with autologous hamstring tendon transplantation.
METHODS: A total of 36 male Beagle dogs were selected, and treated with cut off bilateral anterior cruciate ligament, the tendon of flexor digitorum longus was obtained. Suspended fixation method was used to reconstruct anterior cruciate ligament; which were randomly divided into 3 groups: calcium acid phosphate group, calcium sulfate group and blank group. Calcium acid phosphate was injected into femoral tendon bone tunnel in calcium acid phosphate group. Calcium sulfate was injected into calcium sulfate group. No additional treatment was applied to blank group after ligament reconstruction. Dogs were undergoing general observation, histological examination and biomechanical test at 1, 2, 3, 4, 6 months after reconstruction.
RESULTS AND CONCLUSION: Tendon-bone interface fibronectin in calcium acid phosphate group and calcium sulfate group was significantly better than that in blank group at 1, 2, 3, 4 months after reconstruction of anterior cruciate ligament. There was no significant difference between calcium acid phosphate group and calcium sulfate group. The healing degree in each group was similar after 6 months. In biomechanics, the pull-off strength of tendon-bone interface in calcium acid phosphate group and calcium sulfate group was higher than that in blank group (P < 0.05). However, there was no significance between calcium acid phosphate group and calcium sulfate group (P > 0.05). It is indicated that calcium acid phosphate and calcium sulfate can promote tendon-bone healing, and there was no significant difference between two groups.

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