Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3811-3814.doi: 10.3969/j.issn.1673-8225.2011.21.004

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Adhesion and proliferation of osteoblasts on the surface of titanium alloy modified by chitosan-casein phosphopeptides

Wang Lin1, Bu Yin-zhong1, Wang Jin-qing2, Yang Sheng-rong2, Liu Bin1   

  1. 1School of Stomatology, Lanzhou University, Lanzhou   730000, Gansu Province, China
    2National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou   730000, Gansu Province, China
  • Received:2010-11-09 Revised:2010-12-10 Online:2011-05-21 Published:2011-05-21
  • Contact: Liu Bin, Doctor, Professor, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China liubkq@lzu.edu.cn Correspondence to: Wang Jin-qing, Doctor, Investigator, National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China jqwang@lzb.ac.cn
  • About author:Wang Lin★, Studying for master’s degree, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China walkiewl@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50801065*; 100 Talents Program of the Chinese Academy of Sciences*; Science and Technology Planning Project of Lanzhou Technology Bureau, No. 2008-1-86*; Open Program of National State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No. 0808*

Abstract:

BACKGROUND: The surface of titanium alloy only absorbs some organic molecule to activate the growth factor and stimulate cell proliferation, thereby activating the surrounding bone cells and producing a marked effect.
OBJECTIVE: To prepare a bioactive coating on the surface of the titanium alloy implant, and to evaluate one promising strategy to enhance osseointegration.
METHODS: The surface of titanium alloy modified by silane coupling agent was covalently grafted with chitosan (CS) and the concentration of 0, 5, 10 g/L casein phosphopeptides (CPP) in turn to form composite coatings using multi-step assembly method, compared with unmodified titanium alloy. The biocompatibility of the fabricated coatings was evaluated through co-culture of the titanium alloy and osteoblasts that modified through different procedures, using cell counting, fluorescence staining, and MTT colorimetric assay.
RESULTS AND CONCLUSION: Compared with the titanium alloy without modification, the early adhesion and proliferation of surface of titanium alloy were significantly increased after CS covalently grafted with CPP (P < 0.05). Moreover, with the increase of CPP concentration, the promotion enhanced significantly (P < 0.05). (The osteoblasts on the surface of titanium alloy without modification displayed smooth surface, clear boundary, not fully stretched; the osteoblast cell body of the surface of titanium alloy increased significantly, rough surface, fuzzy boundaries, and fully stretched after modification by CS -CPP composite coating). It is indicated that the prepared CS-CPP composite coating can significantly increased the function of osteoblasts, improve biological properties of titanium alloy, and expected to be an effective method to promote osseointegration.

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