Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3745-3748.doi: 10.3969/j.issn.1673-8225.2011.20.032

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Expression of connective tissue growth factor in the skin wound of diabetic rats

Kuang Yu-zhen, Xiao Xin-hua   

  1. Department of Dermatology, the First Affiliated Hospital, University of South China, Hengyang  421001, Hunan Province, China
  • Received:2011-01-18 Revised:2011-03-15 Online:2011-05-14 Published:2011-05-14
  • Contact: Xiao Xin-hua, Doctor, Associate professor, Department of Endocrinology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China xiaoxinhua139@ 163.com
  • About author:Kuang Yu-zhen★, Master, Attending physician, Department of Dermatology, the First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China kuangyuzhen@ yahoo.com.cn
  • Supported by:

    Science and Technology Committee of Hengyang City, No. 2010kj44*

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Abstract:

BACKGROUND: Studies about connective tissue growth factor (CTGF) expression in the wound healing in diabetes mellitus are rare.
OBJECTIVE: To study CTGF expression during wound healing in diabetic rats induced by streptozotoein.
METHODS: Fifty SD male rats were randomly divided into control group and model group. 50 mg/kg STZ were given intraperitoneally to model rats. After 3 weeks, a round skin of 1.3 cm2 was excised on all dorsal back of rats. The healing time and healing rate were observed according to re-epithelization. Routine HE staining was made to calculate the granulation tissue thickness and angiogenesis at different time points. Western blotting was used to detect the expression of protein of CTGF in the skin at those time points.
RESULTS AND CONCLUSION: The healing time in the model group was (25.06±2.11) days, significantly longer than (16.17±1.88) days in the control group (P  < 0.01). The healing rates in the model group were significantly less than that in the control group at days 4, 8, 12 and 16 (P < 0.01). The amount of granulation tissue thickness and angiogenesis in the model group were significantly less than those in the control group on days 8, 12 and 16, respectively (P  < 0.01). The expression of CTGF protein in the wound of the control group increased with time, the values were much higher than that in the model group after day 12 (P < 0.01). Streptozotocin (STZ)-induced diabetic rats impairs wound healing which is possibly caused by the relative reduce of CTGF expression in the wound compared to non-diabetic rats. The biology of wound healing and the pathobiology of impaired wound healing in diabetes are emphasized to illustrate how these future molecular therapeutics are intended to counteract disease pathology and promote normal wound repair.

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