Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3657-3661.doi: 10.3969/j.issn.1673-8225.2011.20.012

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Proliferation of glial cells and expression of glial fibrillary acidic protein after spinal cord injury in rats

Li Kuan-xin1, 2, Li Feng1   

  1. 1Department of Orthopedics, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China
    2First Department of Orthopedics, the First Affiliated Hospital of Shihezi University Medical School, Shihezi  832000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-01-24 Revised:2011-04-08 Online:2011-05-14 Published:2011-05-14
  • Contact: Li Feng, Chief physician Doctor, Department of Orthopedics, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China lifeng6311@yahoo. com.cn
  • About author:Li Kuan-xin★, Master, Associate chief physician, Department of Orthopedics, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; First Department of Orthopedics, the First Affiliated Hospital of Shihezi University Medical School, Shihezi 832000, Xinjiang Uygur Autonomous Region, China likuanxin@sohu.com

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Abstract:

BACKGROUND: Astrocytes can release various neurotrophic factors by cell lysis to promote spinal cord repair.
OBJECTIVE: To observe the recovery of hjndlimb function and the expression of glial fibrillary acidic protein (GFAP) after spinal cord injury.
METHODS: Spinal cord injury models at T9-10 were established with Allen’s method. After modeling, bone morphogenetic protein 7 was injected into the subarachnoid space. Normal SD rats with His protein injected into the subarachnoid space were used as controls. The behavioral evaluation was performed with the Basso, Beattie & Bresnahanlocomotor rating scale (BBB scale). GFAP expression was detected using hematoxylin-eosin staining and immunohistochemistry staining. 
RESULTS AND CONCLUSION: Based on BBB score, the hindlimb function of rats in the model group recovered 68%. GFAP expression in the model group increased gradually at 3 and 7 days after injury (P < 0.05), and then decreased similar to the control group at 28 days after injury (P > 0.05). The data indicated that injection of bone morphogenetic protein 7 into the subarachnoid space can induce the proliferation of glial cells, increase GFAP expression and then to promote the recovery of hindlimb function after spinal cord injury in rats.

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